枯草芽孢杆菌BZJN1发酵培养基和培养条件的优化  

Optimization of fermentation mediaum and conditions for Bacillus subtilis BZJN1

作　　者：刘芳 廖先清 周荣华 饶犇 陈伟 黄大野 游景茂[2] LIU Fang;LIAO Xian-qing;ZHOU Rong-hua;RAO Ben;CHEN Wei;HUANG Da-ye;YOU Jing-mao(Hubei Biopesticide Engineering Research Centre,Wuhan 430064,China;Institute of Chinese Herbal Medicines,Hubei Academy of Agricultural Sciences,Enshi 445000,Hubei,China)

机构地区：[1]湖北省生物农药工程研究中心,武汉430064 [2]湖北省农业科学院中药材研究所,湖北恩施445000

出　　处：《湖北农业科学》2019年第20期93-96,共4页Hubei Agricultural Sciences

基　　金：湖北省技术创新专项（民族专项2018AKB037）;武汉市应用基础研究计划项目（2017020201010225）;湖北省重大专项（2017ACA175）;湖北省农业科学院青年科学基金项目（2018NKYJJ16）;国家现代农业产业技术体系建设专项（CARS-21）

摘　　要：对枯草芽孢杆菌(Bacillus subtilis)BZJN1发酵培养基及培养条件进行优化。结果表明,BZJN1菌株最适发酵培养基为玉米粉25.0 g/L、鱼粉25.0 g/L、工业蛋白胨15.0 g/L、玉米浆5.0 g/L、碳酸钙1.0 g/L、硫酸镁0.3 g/L、磷酸二氢钾0.1 g/L。最适发酵培养条件为初始pH 7.0、培养温度30℃、接种量4%、转速220 r/min,在此条件下,发酵液芽孢数可达9.68×109 CFU/mL,相比初始基础发酵培养基及培养条件下的发酵水平提高了约2倍。The fermentation media and conditions for the strain of Bacillus subtilis BZJN1 had been optimized.It showed that the composition of the optimized medium was as followings:Corn starch 25.0 g/L,fish meal 25.0 g/L,peptone 15.0 g/L,corn steep liquor 5.0 g/L,CaCO3 1.0 g/L,MgSO4 0.3 g/L and KH2PO4 0.1 g/L.The optimized fermentation condition was as following:Starting pH 7.0,temperature 30℃,inoculum ratio 4%,and stirring speed 220 r/min.The spore counts of the fermentation culture might reach 9.68×109 CFU/mL under the optimized condition with tthe optimized medium,which was about 2 times higher than that in the primary medium and fermentation condition.

关 键 词：枯草芽孢杆菌(Bacillus subtilis) 发酵培养基 条件 优化 

分 类 号：TS201.3[轻工技术与工程—食品科学]