基于质谱流式技术的蛋白组学在肝癌肿瘤异质性的研究  

Study on heterogeneity of hepatocellular xarcinoma by proteomics based on mass spectrometry

作　　者：胡嘉欣 覃朝[3] 陶玉婷[2] 赖智勇 李枝键 齐亚鹏 林星谷[2] 王秋雁 Hu Jiaxin;Qin Zhao;Tao Yuting;Lai Zhiyong;Li Zhijian;Qi Yapeng;Lin Xinggu;Wang Qiuyan(Department of Biochemistry and Molecular Biology,Basic Medical College;Center For Genomic and Personalized Medicine,Guangxi Medical University,Nanning 530021,China;The First Affiliated Hospital of Guangxi Medical University,Nanning 530021,China;The Affiliated Tumor Hospital of Guangxi Medical University,Nanning 530021,China)

机构地区：[1]广西医科大学基础医学院生物化学与分子生物学教研室,南宁530021 [2]广西医科大学基因组与个体化医学研究中心,南宁530021 [3]广西医科大学第一附属医院,南宁530021 [4]广西医科大学附属肿瘤医院,南宁530021

出　　处：《广西医科大学学报》2019年第10期1555-1560,共6页Journal of Guangxi Medical University

基　　金：国家自然科学基金资助项目(No.31560311);广西自然科学基金资助项目(No.2016JJB140183);广西自然科学基金创新团队资助项目(No.2016GXNSFGA380006);广西科技开发课题资助项目(No.桂科AD17195090);广西中青年教师基础能力提升课题资助项目(No.KY2016LX055);广西急诊与医学救援人才小高地课题资助项目(No.GXJZ201611)

摘　　要：目的:利用质谱流式技术建立研究肝细胞癌(HCC)肿瘤异质性的方法。方法:收集肝癌患者样本并制备成单细胞悬液。通过质谱流式技术用15种肿瘤干细胞标志物和28种免疫细胞相关的标志物对肝癌组织及其癌旁组织进行蛋白组学检测。结果:质谱流式分析有效地分出肝癌干细胞样亚群,并将免疫细胞分为25个细胞亚群。肝癌组织与其癌旁组织在肿瘤干细胞表达及免疫微环境组成方面存在差异。结论:质谱流式技术可实现对肿瘤干细胞样细胞和免疫细胞的精细分群,是检测肿瘤异质性的有效方法。Objective:To establish the heterogeneity of hepatocellular carcinoma by mass spectrometry.Methods:We collected and isolated tissue from a case of liver cancer.Proteomics detection of hepatocarcinoma tissues was performed by mass spectrometry using 15 tumor stem cell markers and 28 immune cell-associated markers.Results:Mass spectrometry analysis effectively identified liver cancer stem cells-like subsets and divided the immune cells into 25 cell subpopulations.There was a difference in the expression of tumor stem cells and the composition of immune microenvironment between liver cancer tissues and its adjacent tissues.Conclusion:Mass spectrometry is an effective method to detect tumor heterogeneity by classify hepatocellular carcinoma stem cells and immune cells.

关 键 词：质谱流式技术 肿瘤异质性 肝癌 

分 类 号：R735.7[医药卫生—肿瘤]