肺癌食管癌缺失基因1及其甲基化在鼻内翻性乳头状瘤中的表达研究  

Research of expression of DLEC1 and its methylation in patients with nasal inverted papilloma

作　　者：蒋伟蓉[1] 唐金勇[2] 李云秋 钟宇 Wei-rong Jiang;Jin-yong Tang;Yun-qiu Li;Yu Zhong(Yueyang Vocational Technical College of Hunan Province,Yueyang,Hunan 414000,China;Department of otolaryngology Head and Neck Surgery,The First People’s Hospital of Chenzhou City,Chenzhou,Hunan 423000,China;Department of otolaryngology,Hunan Provincial People’s Hospital,Changsha,Hunan 410005,China)

机构地区：[1]湖南岳阳职业技术学院,湖南岳阳414000 [2]郴州市第一人民医院耳鼻咽喉头颈外科,湖南郴州423000 [3]湖南师范大学附属第一医院耳鼻喉科,湖南长沙410005

出　　处：《中国现代医学杂志》2019年第21期33-36,共4页China Journal of Modern Medicine

基　　金：湖南省科技厅一般项目(No:2013FJ6034)

摘　　要：目的探讨肺癌食管癌缺失基因1(DLEC1)表达及其甲基化在鼻内翻性乳头状瘤(NIP)中表达的临床意义及与Ki-67表达的关系。方法收集30例NIP组织作为NIP组,20例鼻黏膜组织作为对照组,采用甲基化特异性聚合酶链反应(MSP)检测DLEC1基因启动子区域甲基化情况,免疫组织化学法检测DLEC1和Ki-67的表达。结果30例NIP组织中DLEC1基因启动子甲基化比例为43.3%,高于正常组织(2.0%)(P<0.05);NIP组DLEC1失表达率为76.7%,高于对照组45.0%(P<0.05);NIP组Ki-67阳性表达率为60.0%,高于对照组10.0%阳性表达率(P<0.05);NIP组织中DLEC1的表达与Ki-67的表达呈负相关(P<0.05)。结论DLEC1基因启动子甲基化在NIP患者中表达较高,DLEC1在NIP中表达下调且与Ki-67呈负相关,DLEC1表达缺失可能与NIP的发病相关。Objective To investigate the clinical value of DLEC1 gene expression and its methylation in nasal inverted papilloma(NIP)and its relationship with Ki-67 expression.Methods Thirty NSCLC tissues were collected as NIP group and 20 nasal mucosa tissues as control group.Methylation speci fic polymerase chain reaction(MSP)was used to detect the methylation of the DLEC1 gene promoter region.The expression of DLEC1 and Ki-67 was detected by immunohistochemistry.Results The methylation ratio of DLEC1 gene promoter in NIP tissues was 43.3%,which was higher than that in normal tissue(2.0%)(P<0.05).The negative rate of DLEC1 in the NIP group(76.7%)was higher than that in the control group(45.0%)(P<0.05),and the positive rate of Ki-67 in the NIP group(60.0%)was significantly higher than that in the control group(10.0%)(P<0.05);the correlation between the expression of DLEC1 and Ki-67 in NIP tissue was negatively correlated(P<0.05).Conclusions Methylation of DLEC1 gene promoter is highly expressed in NIP patients,and the express of DLEC1 is downregulated in NIP and negatively correlated with Ki-67.

关 键 词：鼻内翻性乳头状瘤 肺癌食管癌缺失基因1 KI-67 甲基化 免疫组织化学 

分 类 号：R739.62[医药卫生—肿瘤]