藤茶源二氢杨梅素体外对破骨细胞的活性  

The Activity of Ampelopsis Grossedentata Source Dihydromyricetin in Vitro on Osteoclasts

作　　者：史倩 魏永忠 周英 王慧娟 陈琳 俸婷婷 SHI Qian;WEI Yongzhong;ZHOU Ying;WANG Huijuan;CHEN Lin;FENG Tingting(School of Liquor and Food Engineering,Guizhou University,Guiyang 550025;Guiyang College of Traditional Chinese Medicine,Guiyang,Guizhou 550025;Research and Development Center of Traditional Chinese Medicine and Natural Medicine of Guizhou University,Guiyang 550025;National&Local Joint Engineering Research Center for the Exploitation and Utilization Technology of Medicine and Food Dual-use Resources in the Southwest of China,Guiyang 550025;Guizhou Engineering Research Center for the Exploitation and Utilization Technology of Medicine and Food Dual-use Resources,Guiyang 550025)

机构地区：[1]贵州大学酿酒与食品工程学院,贵阳550025 [2]贵阳中医学院药学院,贵阳550025 [3]贵州大学中药天然药研发中心,贵阳550025 [4]西南药食两用资源开发利用技术国家地方联合工程研究中心,贵阳550025 [5]贵州省药食同源植物资源开发工程技术研究中心,贵阳550025

出　　处：《食品工业》2019年第9期196-200,共5页The Food Industry

基　　金：贵州省高层次创新型人才(黔科合人才[2015]4032号);贵州省科技计划社会攻关计划项目(黔科合[2016]支撑2909)

摘　　要：试验旨在研究不同浓度二氢杨梅素体外对破骨细胞活性的影响。用MTT方法检测不同浓度的二氢杨梅素对原代骨髓细胞生长存活率的影响。分离新西兰乳兔原代骨髓细胞,用1α, 25-(OH)2 Vit D3诱导获得破骨细胞,以雌二醇(10-8 mol/L)为阳性对照,根据骨吸收陷窝总面积、TRAP+细胞数及TRAP酶活性,分析藤茶源二氢杨梅素对破骨细胞活性的影响。结果表明,当二氢杨梅素浓度为10-5~10-8 mol/L时,骨髓细胞生长存活率接近100%。不同剂量二氢杨梅素对破骨细胞的活性均有抑制作用,其中以10-6和10-7 mol/L的剂量组对破骨细胞活性的抑制效果良好(p<0.01), 10-6 mol/L剂量组对破骨细胞活性的抑制效果最为显著(p<0.01)。藤茶源二氢杨梅素对破骨细胞活性有良好抑制作用。It is to study the effects of different concentrations of dihydromyricetin on osteoclast activity in vitro. The effects of different concentrations of dihydromyricetin on the growth and survival rate of primary bone marrow cells were examined by MTT assay. The primary bone marrow cells of New Zealand milk rabbits were isolated, osteoclasts were induced by 1α, 25-(OH)2 VitD3 using estradiol(10-8 mol/L) as a positive control, and the effect of ampelopsis grossedentata source dihydromyricetin on osteoclast activity was analyzed based on the total area of bone resorption lacuna, TRAP+ cell number and TRAP enzyme activity. The results showed that the growth rate of bone marrow cells with dihydromyricetin concentration of 10-5 to 10-8 mol/L was close to 100%. The different doses of dihydromyricetin inhibited the activity of osteoclasts, and the inhibition of osteoclast activity was good in the dose groups of 10-6 mol/L and 10-7 mol/L(p<0.01). The 10-6 mol/L dose group had the most significant inhibitory effect on osteoclast activity(p<0.01). Dihydromyricetin from ampelopsis grossedentata has a good inhibitory effect on osteoclast activity.

关 键 词：二氢杨梅素 破骨细胞 活性 

分 类 号：S66[农业科学—果树学]