七味白术散对菌群失调腹泻小鼠肠道蔗糖酶活性的影响  

Effect of Qiwei Baizhu San on intestinal sucrase activity in mice with diarrhea

作　　者：郭抗萧 彭昕欣[2] 毛娅男[2] 徐斯盛 杨智英 谭周进[2] 周赛男[2] GUO Kangxiao;PENG Xinxin;MAO Ya′nan;XU Sisheng;YANG Zhiying;TAN Zhoujin;ZHOU Sainan(Changsha Health Vocational College,Changsha,Hu′nan 410100,China)

机构地区：[1]长沙卫生职业学院,湖南长沙410100 [2]湖南中医药大学,湖南长沙410208

出　　处：《中国微生态学杂志》2019年第10期1130-1134,共5页Chinese Journal of Microecology

基　　金：国家自然科学基金(81573951);湖南省教育厅项目(13B082);方剂学湖南省重点学科

摘　　要：目的探究七味白术散对菌群失调腹泻小鼠肠道蔗糖酶活性的影响,为其临床应用提供依据。方法将132只SPF级KM小鼠分为正常组、模型组、七味白术散组,每组44只,雌雄各半。采用硫酸庆大霉素和头孢拉定混合抗生素制备腹泻小鼠模型,造模成功后,七味白术散组给予七味白术散汤剂进行治疗,正常组和模型组给予等量无菌水进行灌胃,连续治疗6d。造模结束和治疗期间每天灌胃前进行小鼠肠道取材,分别测定小鼠肠道内容物、肠道黏膜前中后段蔗糖酶活性,观察治疗期间蔗糖酶活性的动态变化。结果造模结束后,小鼠的肠道内容物和肠道黏膜前中后段蔗糖酶活性与正常组比均下降明显(t=23.684,P<0.01)。肠道内容物:治疗第1天,模型组、七味白术散组蔗糖酶活性仍低于正常组(t=13.909,P<0.01),到治疗第3天,模型组和正常组差异无统计学意义(P>0.05),七味白术散组显著高于正常组和模型组(t=14.189,P<0.01)。肠道黏膜前段和中段:治疗第1天,模型组和七味白术散组酶活性差异无统计学意义(P>0.05),且均低于正常组(t=19.274,P<0.01),治疗第3天,模型组和正常组酶活性差异无统计学意义(P>0.05),七味白术散组酶活性远远高于正常组和模型组(t=19.467,P<0.01)。黏膜后段:治疗第1天,模型组和七味白术散组酶活性差异无统计学意义(P>0.05),均显著低于正常组(t=12.783,P<0.01),治疗第3天,模型组和正常组酶活性差异无统计学意义(P>0.05),七味白术散组酶活性均远远高于正常组和模型组(t=10.942,P<0.01)。结论抗生素可引起小鼠腹泻及肠道蔗糖酶活性下降,七味白术散可促进腹泻小鼠肠道黏膜蔗糖酶活性恢复而治疗腹泻。Objective To explore the activity of sucrase in the intestine of mice with dysbacteriosis diarrhea and provide reference for rational clinical application of Qiwei Baizhu San.Methods A total of 132mice were randomly divided into normal group,model group,Qiwei Baizhu San(QBS)group or lactase group.All groups except the normal group were intragastrically administered antibiotics for 5days to establish dysbacteriosis diarrhea models,then treated with Qiwei Baizhu San and lactase respectively.The normal group and model group were given the same amount of sterile water for 6days.The intestines of mice were taken before perfusion every day to detect the activity of sucrose in the intestinal contents and the anterior,middle and posterior segments of intestinal mucosa,and the dynamic changes of invertase activity during the treatment were observed.Results After modeling,the activities of sucrose in the intestinal contents and the anterior,middle and posterior segments of intestinal mucosa decreased significantly compared with the normal group(t=23.684,P<0.01).Intestinal contents:the invertase activities in the model group,QBS group and lactase group were lower than in normal group(t=13.909,P<0.01)at the first day of treatment.There was no difference between the model group and normal group at the third day of treatment(P>0.05);QBS group and lactase group were significantly superior to the normal group and model group(t=14.189,P<0.01).Anterior and middle segments of intestinal mucosa:there was no difference in enzyme activity between the model group and QBS Group(P>0.05)at the first day of treatment,and the activities in the two groups were lower than in normal group and lactase group(t=19.274,P<0.01).There was no difference in enzyme activity between the model group and normal group(P>0.05)at the third day of treatment;QBS group and lactase group were superior to the normal group and model group(t=19.467,P<0.01).Posterior mucosa:there was no difference in enzyme activity between the model group and lactase group(P>0.05)a

关 键 词：七味白术散 菌群失调腹泻 小鼠 蔗糖酶 

分 类 号：R9[医药卫生—药学]