苦参提取物对口腔主要致龋细菌作用的实验研究  

The effect of Sophora flavescens Ait extract on the main cariogenic bacteria in oral cavity

作　　者：从兆霞 袁曦玉 吴泽钰 韩婧 赵今[1,2] CONG Zhaoxia;YUAN Xiyu;WU Zeyu;HAN Jing;ZHAO Jin(The First Affiliated Hospital of Xinjiang Medical University(Affiliated Stomatological Hospital),Urumqi,Xinjiang 830000,China)

机构地区：[1]新疆医科大学第一附属医院(附属口腔医院),新疆乌鲁木齐830000 [2]新疆维吾尔自治区口腔医学研究所,新疆乌鲁木齐830000

出　　处：《中国微生态学杂志》2019年第10期1186-1192,共7页Chinese Journal of Microecology

基　　金：国家自然科学基金(81760194)

摘　　要：目的观察苦参提取物对口腔主要致龋细菌及其生物膜生长、黏附、产酸和产糖的影响,探寻其防龋作用机制。方法将苦参提取物按照二倍梯度稀释法测定最低抑菌浓度,0.5g/L氯己定为阳性对照,不含药液组为阴性对照组;采用紫外分光光度计测定细菌黏附能力;通过生物膜结晶紫染色法测定生物膜抑制浓度和生物膜清除浓度;通过ΔpH法和苯酚-硫酸法分别测定细菌的产酸和合成水不溶性胞外多糖情况。结果苦参提取物对口腔主要致龋细菌的最低抑菌浓度均为4g/L;在4g/L时,对变形链球菌、远缘链球菌、血链球菌、粘性放线菌和内氏放线菌黏附抑制率分别为(77.6%±1.2%)、(66.7%±1.8%)、(60.68%±2.9%)、(79.8%±1.2%)和(85.1%±1.3%)。2g/L时能够显著抑制浮游菌产酸及合成水不溶性胞外多糖能力。4g/L时对变形链球菌、远缘链球菌、血链球菌、粘性放线菌、内氏放线菌和嗜酸乳杆菌生物膜形成抑制率分别为(87.5%±1.3%)、(85.4%±0.5%)、(89.0%±0.3%)、(77.2%±0.7%)、(87.4%±1.1%)和(80.4%±1.3%);并对以上细菌生物膜的最低清除浓度分别为16、16、16、16、8和8g/L。苦参提取物在50%的最小生物膜清除浓度下对单菌生物膜的产酸和合成水不溶性细胞外多糖的抑制率分别为67.5%~94.1%和42.3%~60.0%。结论苦参提取物能够抑制口腔主要致龋细菌浮游和生物膜状态下的生长、黏附、产酸和产糖,其有望成为一种龋齿预防制剂。Objective To observe the effects of Sophora flavescens Ait extract on the growth,adhesion,acid production and sugar production of the main cariogenic bacteria and their biofilms,and explore its anti-caries mechanism.Methods The minimum inhibitory concentration of the extract of SophoraflavescensAitwas determined by double gradient dilution method,with 0.5g/L chlorhexidine as the positive controls while the drug-free group as the negative controls.The bacterial adhesion was determined using ultraviolet spectrophotometer.The biofilm inhibition concentration and biofilm removal concentration were determined with membrane crystal violet staining;bacterial acid production and synthesis of water insoluble extracellular polysaccharides were determined withΔpH method and phenol-sulfuric acid method,respectively.Results The minimum inhibitory concentration of Sophora flavescens Ait extract to the main cariogenic bacteria was 4g/L,at which the adhesion inhibition rates for Streptococcus mutans,Streptococcus sobrinus,Streptococcus sanguis,Actinomyces viscosus and Aetinomyces naeslundii were(77.6%±1.2%),(66.7%±1.8%),(60.68%±2.9%),(79.8%±1.2%)and(85.1%±1.3%),respectively.At 2g/L,the extract significantly inhibited the ability of planktonic bacteria to produce acid and synthesize water-insoluble extracellular polysaccharides.The inhibition rate of biofilm formation of Streptococcus mutans,Streptococcus sobrinus,Streptococcus sanguis,Actinomyces viscosus,Aetinomyces naeslundii and Lactobacillus acidophilus at 4g/L was(87.5%±1.3%),(85.4%±0.5%),(89.0%±0.3%),(77.2%±0.7%),(87.4%±1.1%)and(80.4%±1.3%),respectively.The minimum biofilm eradication concentrations to the above bacteria were 16g/L,16g/L,16g/L,16g/L,8g/L and 8g/L,respectively.At 50%minimum biofilm eradication concentration,the inhibition rates of Sophora flavescens Ait extracts to acid production and synthesis of water-insoluble extracellular polysaccharides in single biofilms were 67.5%to 94.1%and 42.3%to 60.0%,respectively.Conclusion Sophora flavescens Ait ex

关 键 词：苦参 致龋细菌 生物膜 毒力因子 

分 类 号：R780.1[医药卫生—口腔医学]