基于Cu-TPA的电化学生物传感器对黄曲霉毒素B1的检测  

An Electrochemical Biosensor Based on Cu-TPA for Determination of Aflatoxin B1

作　　者：王春燕 蒋晓青 周泊 WANG Chunyan;JIANG Xiaoqing;ZHOU Bo(School of Chemistry and Materials Science,Jiangsu Key Laboratory of New Power Batteries,Jiangsu Collaborative Innovation Center of Biomedical Functional Materials,Jiangsu Key Laboratory of Biomedical Materials,Nanjing Normal University,Nanjing 210023,China)

机构地区：[1]南京师范大学化学与材料科学学院江苏省新型动力电池重点实验室江苏省生物医药功能材料协同创新中心江苏省生物医学材料重点实验室

出　　处：《高等学校化学学报》2019年第11期2301-2307,共7页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金(批准号:21175068)资助~~

摘　　要：利用对苯二甲酸铜(Cu-TPA)能产生强的电化学信号设计了一种灵敏的电化学生物传感器,并将其用于测定黄曲霉毒素B1(AFB1).信号探针中的Cu-TPA含有可产生电化学信号的Cu(Ⅱ),当加入一定量的AFB1后,AFB1与探针中特定的适配体结合,使信号探针脱落,电化学信号降低.根据电化学信号值的变化实现了对AFB1的检测.在最佳条件下,该传感器的检出限为4.2×10^-6ng/m L(S/N=3),线性范围为10^-5~10 ng/m L.将该传感器用于啤酒中AFB1的检测,回收率为95%~106%.Copper terephthalate(Cu TPA),which could produce strong electrochemical signal,was used as a signal probe to design sensitive electrochemical biosensor for determination of aflatoxin B1(AFB1).Cu TPA/AuNPs composites contain large amounts of Cu(Ⅱ),which can obtain strong electrochemical signal.When a certain amount of AFB1 was added,AFB1 and AFB1 aptamer combined to make the signal probe fall off and the electrochemical signal reduced.Under the optimized conditions,this electrochemical biosensor for AFB1 exhibited low detection limit of 4.2×10^-6 ng/mL(S/N=3)and wide linear range of 10^-5-10 ng/mL.The biosensor was also used to analyze AFB1 spiked beer sample and the recovery rate was 95%-106%.

关 键 词：对苯二甲酸铜 适配体 黄曲霉毒素B1 电化学生物传感器 

分 类 号：O657.1[理学—分析化学] O646[理学—化学]