A GmNINa-miR172c-NNC1 Regulatory Network Coordinates the Nodulation and Autoregulation of Nodulation Pathways in Soybean  

作　　者：Lixiang Wang Zhengxi Sun Chao Su Yongliang Wang Qiqi Yan Jiahuan Chen Thomas Ott Xia Li 

机构地区：[1]State Key Laboratory of Agricultural Microbiology,College of Plant Science and Technology,Huazhong Agricultural University,No.1 Shizishan Road,Hongshan District,Wuhan,Hubei 430070,P.R.China [2]College of Biological Science and Engineering,Panzhihua University,No.10 Airport Road,Eastern District,Panzhihua,Sichuan,China [3]University of Freiburg.Faculty of Biology.University of Freiburg,Schdnz丨estrasse 1,79104 Freiburg.Germany [4]CIBSS-Centre for Integrative Biological Signalling Studies,University of Freiburg,79104 Freiburg,Germany

出　　处：《Molecular Plant》2019年第9期1211-1226,共16页分子植物（英文版）

基　　金：the National Key Research and Development Program of China(2016YFA0500503);the National Natural Science Foundation of China(31730066 and 31230050);the Ministry of Agriculture of the People's Public of China(2018ZX0800919B and 2014ZX0800929B);Huazhong Agricultural University Scientific&Technological Selfinnovation Foundation(2015RC014).

摘　　要：Symbiotic root nodules are root lateral organs of plants in which nitrogen-fixing bacteria(rhizobia)convert atmospheric nitrogen to ammonia.The formation and number of nodules in legumes are precisely controlled by a rhizobia-induced signal cascade and host-controlled autoregulation of nodulation(AON).However,how these pathways are integrated and their underlying mechanisms are unclear.Here,we report that microRNA172c(miR172c)activates soybean(Glycine max)R hizobia-induced CLE1(GmRICI)and GmRIC2 by removing the transcriptional repression of these genes by Nodule Number Control 1(NNC1),leading to the activation of the AON pathway.NNC1 interacts with GmNINa,the soybean ortholog of Lotus NODULE INCEPTION(NIN),and hampers its transcriptional activation o i G m RICI and GmRIC2.Importantly,GmNINa acts as a transcriptional activator of miR172c.Intriguingly,NNC1 can transcriptionally repress miR172c expression,adding a negative feedback loop into the NNC1 regulatory network.Moreover,GmNINa interacts with NNC1 and can relieve the NNC1-mediated repression of miR172c transcription.Thus,the GmNINa-miR172c-NNC1 network is a master switch that coordinately regulates and optimizes NF and AON signaling,supporting the balance between nodulation and AON in soybean.

关 键 词：NODULE number AUTOREGULATION of NODULATION miR172c-NNC1 negative feedback loop NODULE INCEPTION 

分 类 号：TP3[自动化与计算机技术—计算机科学与技术]