SOX10作为原发性口腔黏膜恶性黑色素瘤分子诊断指标的初步研究  

A Preliminary Study on SOX10 as A Molecular Diagnostic Marker for Primary Oral Mucosal Melanoma

作　　者：马旭辉 王丽珍[2] 任国欣 吴云腾 顾挺 郭伟 MA Xu-hui;WANG Li-zheng;REN Guo-xin;WU Yun-teng;GU Ting;GUO Wei(Department of Oromaxillofacial Head and Neck Oncology,Shanghai Jiaotong University School of Medicine,National Clinical Research Center for Oral Diseases,Shanghai Key Laboratory of Stomatology,Shanghai Research Institute of Stomatology,Shanghai 200011,China;Department of Oral Histopathology,Shanghai Ninth People's Hospital,College of Stomatology,Shanghai Jiaotong University School of Medicine,National Clinical Research Center for Oral Diseases,Shanghai Key Laboratory of Stomatology,Shanghai Research Institute of Stomatology,Shanghai 200011,China)

机构地区：[1]国家口腔疾病临床研究中心,上海市口腔医学重点实验室,上海市口腔医学研究所,上海交通大学医学院附属第九人民医院·口腔医学院口腔颌面-头颈肿瘤科 [2]国家口腔疾病临床研究中心,上海市口腔医学重点实验室,上海市口腔医学研究所,上海交通大学医学院附属第九人民医院·口腔医学院口腔颌面-口腔病理科,上海200011

出　　处：《口腔颌面外科杂志》2019年第3期131-136,共6页Journal of Oral and Maxillofacial Surgery

基　　金：国家重点研发计划项目(2016YFC0905003、2016YFC0905000;上海交通大学交叉医学(理)基金(ZH2018QNA09)

摘　　要：目的:阐明SOX10在口腔黏膜恶性黑色素瘤的表达特点。方法:通过免疫组织化学染色对89例口腔黏膜恶性黑色素瘤(OMM)及4例口腔黏膜促结缔组织增生型黑色素瘤(ODM)的石蜡标本中,S-100、HMB-45、Melan-A以及SOX10的表达特点进行分析。结果:在OMM中Melan-A、HMB-45、S-100以及SOX10的表达水平分别为96.6%、93.3%、97.8%及98.8%。在OMM中SOX10的表达水平与S-100、Melan-A无明显统计学差异(P>0.05),但显著高于HMB-45(P<0.05)。在4例ODM中,SOX10与S-100均表达,Melan-A及HMB-45表达均阴性。在OMM及ODM中,SOX10的染色模式均呈强且特异的核染。结论:SOX10可在S-100、Melan-A,HMB-45等常规免疫组化标记物的基础上,作为OMM及ODM病理诊断的有力补充,具有重要的临床应用价值。Objective:To elucidate the expression of SOX10 in primary oral mucosal melanoma(OMM).Methods:The expression of S-100,HMB-45,Melan-A and SOX10 in 89 OMM and 4 ODM(oral mucosal desmoplastic melanoma)in paraffin specimens were analyzed by immunohistochemical staining.Results:In OMM,the expression levels of Melan-A,HMB-45,S-100 and SOX10 were 96.6%,93.3%,97.8%and 98.8%,respectively.The expression level of SOX10 in OMM was not significantly different from S-100 and Melan-A(P>0.05),but significantly higher than that of HMB-45(P<0.05).In 4 ODM cases,SOX10 and S-100 were expressed,while Melan-A and HMB-45 were negative.In OMM and ODM,the staining pattern of SOX10 was strong and specific was present in nuclear.Conclusions:SOX10 could be used as a helpful marker for pathological diagnosis of OMM and ODM on the basis of routine immunohistochemical markers,such as S-100,Melan-A and HMB-45,and is of significant clinical importance.

关 键 词：SOX10 口腔黏膜恶性黑色素瘤 分子诊断 组织病理学 免疫组织化学 

分 类 号：R782.4[医药卫生—口腔医学]