miRNA-31对糖尿病小鼠来源骨髓间充质干细胞体外成骨分化的影响  

miRNA-31 Involved in the Suppressed Osteogenic Differentiation of Bone Marrow Mesenchymal Stem Cells in Diabetes Mellitus

作　　者：范德生[1] 甄蕾 汪黎明 FAN De-sheng;ZHEN Lei;WANG Li-ming(Department of Pathology,Baoshan Branch,Shuguang Hospital,Shanghai University of Traditional Chinese Medicine,Shanghai 201901;Department of Periodontology,Shanghai Stomatological Hospital,Fudan University,Shanghai 200001;Oral Biomedical Engineering Laboratory,Shanghai Stomatological Hospital,Fudan University,Shanghai 200001)

机构地区：[1]上海中医药大学附属曙光医院宝山分院病理科,上海201901 [2]复旦大学附属口腔医院(筹)·上海市口腔病防治院牙周科 [3]复旦大学附属口腔医院(筹)·上海市口腔病防治院口腔生物医学工程实验室,上海200001

出　　处：《口腔颌面外科杂志》2019年第3期137-141,共5页Journal of Oral and Maxillofacial Surgery

基　　金：国家自然科学基金青年项目(81700981);上海市卫计委面上项目(201640103);上海市宝山区科学技术委员会科技创新专项资金项目(18-E-9)

摘　　要：目的:探讨miRNA-31这一微小RNA对糖尿病小鼠来源骨髓间充质干细胞(BMSCs)体外成骨分化的影响。方法:采用高糖高脂饮食加链脲佐菌素腹腔注射诱导建立Ⅱ型糖尿病小鼠模型,造模8周后,在体外原代分离培养BMSCs,检测其miRNA-31和成骨分化情况,并与正常对照组比较。再将糖尿病小鼠来源的BMSCs体外分别转染miRNA-31模拟物和miRNA-31抑制剂,再检测比较2种BMSCs的体外成骨分化能力。结果:糖尿病小鼠造模成功8周后,micro CT检测结果显示与正常对照组小鼠相比,模型组小鼠明显骨密度降低,呈现典型骨质疏松。将糖尿病小鼠来源BMSCs体外培养后,发现与对照组相比,其成骨分化能力降低,miRNA-31表达升高。而转染miRNA-31抑制剂后可部分解除糖尿病微环境对BMSCs成骨分化的抑制。结论:糖尿病微环境下BMSCs高表达miRNA-31,并可抑制BMSCs的成骨分化。Objective:To investigate the effect of miRNA-31 on the osteogenic differentiation of BMSCs in high-glucose microenvironment.Methods:Diabetic osteoporosis(DOP)mice model was established by high glucose and high fat diet plus STZ intraperitoneal injection.Bone marrow mesenchymal stem cells(BMSCs)were isolated and cultured in vitro.The expression of miRNA-31 were detected in vitro.The osteogenic differentiation ability was detected after miRNA-31 mimics and miRNA-31 inhibitors were transfected respectively.Results:DOP model was successfully established.The results of micro-CT showed that DOP mice had lower bone mineral density.BMSCs isolated from DOP model showed lower osteogenic differentiation ability and higher expression of miRNA-31.After transfection of miRNA-31 inhibitors,the osteogenic differentiation ability of BMSCs could be partially restored.Conclusions:BMSCs in hyperglycemia microenvironment will high express miRNA-31,which may inhibit osteogenic differentiation of BMSCs.

关 键 词：糖尿病性骨质疏松症 骨髓间充质干细胞 成骨分化 MICRORNA 

分 类 号：R782.4[医药卫生—口腔医学]