牡丹花蕊醇提物对H2O2诱导HUVEC细胞损伤的保护作用  

Protective effects of alcohol extraction from peony stamen on H2O2-induced damage in HUVEC

作　　者：罗磊[1,2] 关宁宁 向进乐[1,2] 朱文学 LUO Lei;GUAN Ning-ning;XIANG Jin-le;ZHU Wen-xue(College of Food and Bioengineering,Henan University of Science and Technology,Luoyang,Henan 471023,China;Henan Agricultural Products Drying Equipment Engineering Technology Research Center,Luoyang,Henan 471023,China)

机构地区：[1]河南科技大学食品与生物工程学院,河南洛阳471023 [2]河南省农产品干燥装备工程技术研究中心,河南洛阳471023

出　　处：《食品与机械》2019年第10期97-102,共6页Food and Machinery

基　　金：河南省科技攻关项目（编号：172102110019）;河南省高校科技创新团队计划（编号：17IRTSTHN0l6)

摘　　要：采用高效液相色谱分析牡丹花蕊醇提物的主要成分,并通过H2O2诱导HUVEC细胞建立损伤模型,研究牡丹花蕊醇提物对其保护作用。结果表明:牡丹花蕊醇提物主要成分为芦丁、槲皮素和芍药苷3种单体,含量分别为44.25%,15.50%,17.00%;牡丹花蕊醇提物能够降低细胞及其培养液中MDA含量,提高细胞内SOD和GPX活性以及GSH的含量。牡丹花蕊醇提物能够提高HUVEC细胞的抗氧化能力。The main components of the alcohol extraction from peony stamen was analyzed by high performance liquid chromatography.H 2 O 2 induced human umbilical vein endothelial cells(HUVEC)cells to establish a damage model to study the protective effect of peony flower extract.Results:the alcohol extraction from peony stamen mainly contain three monomers,namely rutin,quercetin and paeoniflorin,with contents of 44.25%,15.50%and 17.00%,respectively.The alcohol extraction from peony stamen can reduce the content of MDA in cells and culture medium,increase the activities of SOD,GPX and GSH content in the calls.The alcohol extraction of peony stamen can improve the antioxidant capacity of HUVEC.

关 键 词：牡丹花 醇提物 高效液相色谱法 HUVEC细胞 H2O2损伤 

分 类 号：R28[医药卫生—中药学]