白细胞介素1β对结直肠癌细胞HCT116迁移能力和上皮-间质转化的影响  被引量:4

Effect of IL-1β on human colorectal cancer cell HCT116 metastasis and EMT transformation

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作  者:石斌 樊平 许方方 SHI Bin;FAN Ping;XU Fangfang(Department of General Surgery,the First Affiliated Hospital of USTC(Anhui Provincial Hospital),Hefei 230001,China)

机构地区:[1]中国科学技术大学附属第一医院(安徽省立医院)普外科

出  处:《安徽医学》2019年第10期1079-1083,共5页Anhui Medical Journal

基  金:安徽省自然科学基金项目(项目编号:1608085QH217)

摘  要:目的研究白细胞介素1β(IL-1β)诱导结直肠癌细胞HCT116转移的分子机制。方法HCT116细胞分为空白对照组(不进行任何处理),IL-1β处理组(加入200 pmol/L IL-1β处理),干扰阴性对照+IL-1β处理组(转染阴性干扰对照再加入IL-1β处理),干扰锌指绑定增强蛋白1(ZEB1)+IL-1β处理组(转染ZEB1干扰RNA)。使用Transwell迁移实验研究各组细胞的迁移能力。荧光定量聚合酶监链式反应(PCR)检测各组细胞中E-cadherin和ZEB1基因的表达。免疫印迹实验检测各组细胞E-cadherin和ZEB1蛋白的表达。结果空白对照组穿过Transwell小室的细胞数量为(58.3±7.2)个,IL-1β处理组为(129.6±12.1)个,差异有统计学意义(P<0.05);干扰阴性对照+IL-1β处理组穿过Transwell小室的细胞数量为(108.7±15.9)个,干扰ZEB1+IL-1β处理组为(68.2±11.4)个,差异有统计学意义(P<0.05)。空白对照组HCT116细胞中E-cadherin基因的相对表达量、E-cadherin蛋白相对灰度值均高于IL-1β处理组,差异均有统计学意义(P<0.05);干扰阴性对照+IL-1β处理组HCT116细胞中E-cadherin基因的相对表达量、E-cadherin蛋白相对灰度值均低于干扰ZEB1+IL-1β处理组,差异均有统计学意义(P<0.05)。结论IL-1β通过诱导ZEB1表达促进结直肠癌细胞HCT116发生EMT转化并提高其迁移能力。Objective To investigate the molecular mechanism of interleukin 1β(IL-1β)-mediated metastasis of colorectal cancer cells.Methods HCT116 cells were divided into control group(without treatment),IL-1βgroup(IL-1βtreatment),si-negative+IL-1βgroup(trans fection with si-negative RNAi+IL-1βtreatment)and si-ZEB1+IL-1βgroup(transfection with si-ZEB1 RNAi+I L-1βtreatment).Transwell assay was employed to detect the metastasis capability of each group cells.qRT-PCR was used to investigate the mRNA expression of E-cadherin and ZEB1 in each group cells.Western blot was applied to detect the protein expression of E-cadherin and ZEB1 in each group cells.Results The number of Transwell in control group was smaller than that in IL-1βgroup[(58.3±7.2)vs(129.6±12.1)],and the difference was statistically significant(P<0.05).The number of Transwell of si-ZEB1+IL-1βgroup was smaller than that in si-negative+IL-1βgroup[(68.2±11.4)vs(108.7±15.9)],and the difference was statistically significant(P<0.05).The mRNA expression of E-cadherin in IL-1βgroup was lower than that in control group,and the mRNA expression of E-cadherin in si-ZEB1+IL-1βgroup was higher than that in si-negative+IL-1βgroup,all with statistical significance(P<0.05).The protein expression of E-cadh erin in IL-1βgroup was lower than that in control group,and protein expression of E-cadherin in si-ZEB1+IL-1βgroup was higher than that in si-negative+IL-1βgroup,all with statistical significance(P<0.05).Conclusions IL-1βinduces EMT transformation and metastasis of human colorectal cancer cell HCT116 by up-regulation of ZEB1.

关 键 词:结直肠癌 白细胞介素1Β 上皮间质转化 锌指绑定增强蛋白1 侵袭转移 

分 类 号:R73[医药卫生—肿瘤]

 

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