煅烧异种骨用于犬牙槽嵴保存的初步研究  

Preliminary study on true bone ceramics for alveolar ridge preservation in dogs

作　　者：李晶[1] 乔玮 任晓琦 石浩 杨婷[1] 马绍英[1] 赵亚平[1] 苏成忠[1] LI Jing;QIAO Wei;REN Xiaoqi;SHI Hao;YANG Ting;MA Shaoying;ZHAO Yaping;SU Chengzhong(Shanxi Province Tissue Bank of China Institute for Radiation Protection,Shanxi Osteorad Biomaterial Co.Ltd,Taiyuan Shanxi,030006,P.R.China)

机构地区：[1]中国辐射防护研究院生物材料研发中心山西奥瑞生物材料有限公司

出　　处：《中国修复重建外科杂志》2019年第11期1452-1456,共5页Chinese Journal of Reparative and Reconstructive Surgery

摘　　要：目的研究将牛骨经过高温煅烧制备的煅烧异种骨材料(以下简称煅烧异种骨)用于犬拔牙窝牙槽嵴位点保存的效果。方法取健康1.5~2岁龄比格犬6只,拔除双侧下颌第2、4前磨牙以及上颌第2前磨牙,左侧拔牙窝植入煅烧异种骨作为实验组,右侧植入天然煅烧骨修复材料(商品名:骼瑞)作为对照组。术后1、6个月行伤口及植骨材料大体观察后,分别过量麻醉处死3只实验动物,分离上下颌骨后行锥形束CT检查,测量植骨部位与相邻参照区(邻近的第3前磨牙两个牙根中间的骨区)的平均灰度值,计算植骨处与参照区的灰度比;常规行HE染色观察两组植入材料吸收情况和新骨形成情况,评价材料成骨效果。结果大体观察示,术后2周实验组和对照组创口均基本愈合,植骨材料无裸露;术后1、6个月创口均愈合良好,无肿胀。锥形束CT检查示,术后1个月,两组植骨区均可见植骨材料残留;术后6个月,两组植骨区未见明显植骨材料残留,植骨区牙槽嵴高度均未见明显降低,实验组和对照组牙槽窝内骨量无明显差异。术后1、6个月实验组植骨处与对应参照区的灰度比分别为0.97±0.14、0.93±0.06,对照组分别为0.99±0.16、0.94±0.05,两组比较差异均无统计学意义(t=-1.030,P=0.333;t=-0.770,P=0.466)。HE染色示,术后1个月,两组植骨部位均可见大量植骨材料未降解,植骨材料周围可见新生骨组织;术后6个月,两组植骨材料基本完全降解,均可见大量成骨。结论煅烧异种骨材料能够保持牙槽骨密度,并有良好的成骨效果,能够用于牙槽嵴位点保存。Objective To study the preservation effect of true bone ceramics(TBC) prepared by hightemperature calcination of bovine bone on alveolar ridge of canine extraction socket. Methods Six healthy Beagle dogs(aged 1.5-2 years) were selected to extract the second and fourth premolars of both mandibles and the second premolars of the maxilla. The left extraction socket was implanted with TBC as the experimental group, and the right side was implanted with the calcined bovine bone(CBB) as the control group, to observe the alveolar ridge preservation effect.Three dogs were euthanized after general observation at 1 and 6 months after operation respectively. After separating the maxilla and mandible, cone beam CT(CBCT) was performed to measure the average gray value of the graft site and the adjacent reference area(the area between the roots of the adjacent third premolar) and calculate the gray scale ratio between the bone graft site and the reference area. Histological observation was made on the bone graft site to evaluate the new bone formation. Results General observation showed that the wounds of both groups were basically healed at 2 weeks after operation, and the bone graft materials were not exposed. The wounds healed well at 1 and 6 months after operation without swelling. The results of CBCT showed that the residual material was found in both groups at 1 month after operation, and no significant residual material was found in both groups at 6 months after operation, and the alveolar ridge height of the bone graft area was not significantly reduced. There was no significant difference in the bone mineral density between the experimental group and the control group. The gray scale ratios of the experimental group at 1 month and 6 months after operation were 0.97±0.14 and 0.93±0.06, respectively, and were 0.99±0.16 and 0.94±0.05 in control group, showing no significant difference between the two groups(t=-1.030, P=0.333;t=-0.770, P=0.466). HE staining observation showed that a large number of bone graft material

关 键 词：煅烧异种骨 犬拔牙窝填充 灰度比 牙槽嵴保 

分 类 号：R78[医药卫生—口腔医学]