橡胶树乙醇酸氧化酶蛋白理化特性及基因表达模式分析  

Characterization and Expression Analysis of Glycolate Oxidase from Rubber Tree

作　　者：龙翔宇[1] 卢基来 方永军[1] 秦云霞[1] Long Xiangyu;*Lu Jilai;Fang Yongjun;Qin Yunxia(Rubber Research Institute,Chinese Academy of Tropical Agricultural Sciences,Haikou,571101;Institute of Tropical Agriculture and Forestry,Hainan University,Haikou,571737)

机构地区：[1]中国热带农业科学院橡胶研究所,海口571101 [2]海南大学热带农林学院,海口571737

出　　处：《分子植物育种》2019年第20期6626-6631,共6页Molecular Plant Breeding

基　　金：国家自然科学基金面上项目(31770709);海南省自然科学基金(317279);中央级公益性科研院所基本科研业务费专项(NO.17CXTD-28;NO.1630022017003;NO.1630022019016)共同资助

摘　　要：植物乙醇酸氧化酶(glycolate oxidase, GLO)作为光呼吸作用的关键酶,影响光合组织的碳固定,并参与生物与非生物胁迫应答。本研究基于橡胶树基因组数据库平台,扫描并获得橡胶树GLO基因4个,均有植物GLO的典型特性,分子量约40 ku,二级结构均以α螺旋为主,定位于过氧化物酶体,除含有碱性亚基Ⅰ类外,还有中性亚基Ⅱ类,聚类分析结果也暗示植物GLO进化可能早于物种间进化;橡胶树GLO存在叶片特异表达,HbGOX1为叶片GLO关键成员,随叶片发育表达丰度升高,而受高温胁迫下调表达。本研究结果初步揭示橡胶树GLO存在叶片组织特异性,且通过调节H2O2,参与抗性诱导,同时防止活性氧积累和伤害,有助于解析其在光呼吸中的作用机制,对更系统了解植物光呼吸代谢过程及其调控具有重要意义。In plant, the glycolate oxidase(GLO) is a key enzyme of photorespiration, involving carbon fixation and environment stress. In this study, four GLO genes were detected and analyzed from genome database of rubber tree. They had typical feature of GLO, including 40 ku molecular weight, main alpha helix second structure, and location in peroxisome. They were clearly divided into alkaline and neutral subunits by clustering analysis, indicating their differentiation probably was earlier than plants diverge. They had tissue specific expression, and HbGOX1 was as key members of GLO in leaf. The expression of HbGOX1 was up-regulated following leaf development, and down-regulated under high temperature treatment. These results indicated that GLO have an important role in resistance, preventing accumulation and damage of active oxygen with regulation of H2 O2. It is helpful for understanding mechanism and regulation of photorespiration in plant.

关 键 词：橡胶树 光呼吸 乙醇酸氧化酶 理化性质 表达模式 

分 类 号：Q94[生物学—植物学]