非小细胞肺癌患者外周血白细胞端粒DNA长度与肿瘤类型及预后的关系  

Relationship between telomere DNA length of peripheral leukocytes and tumor type and prognosis in patients with non-small cell lung cancer

作　　者：吴瑞锋[1] 王律 高璐 李良[1] 钟理[2] 张毅[2] 高丛 韩小勇 Wu Ruifeng;Wang Lyu;Gao Lu;Li Liang;Zhong Li;Zhang Yi;Gao Cong;Han Xiaoyong(Department of Thoracic Surgery,Baoding First Central Hospital,Baoding 071002,China;College of Life Sciences,Hebei University,Baoding 071002,China)

机构地区：[1]河北省保定市第一中心医院胸外科,071002 [2]河北大学生命科学院,河北省保定市071002

出　　处：《中国综合临床》2019年第6期481-485,共5页Clinical Medicine of China

基　　金：国家自然科学基金资助项目(81773178)。

摘　　要：目的探讨非小细胞肺癌中外周血白细胞端粒DNA长度与肿瘤类型及预后之间的关系。方法纳入2016年6月至2017年4月河北省保定市第一中心医院胸外科经病理学检查确诊为肺癌并有明确TNM分期患者,排除一般情况记录不详、失访患者,最终纳入患者共55例,其中腺癌组35例,其他非小细胞肺癌组20例。采用实时荧光定量PCR法对非小细胞肺癌患者外周血白细胞端粒DNA长度进行检测,并随访患者生存情况,分析外周血白细胞端粒DNA长度与肿瘤病理类型及患者预后的关系。结果腺癌组的端粒DNA长度(T/S)1.98±0.69大于其他非小细胞肺癌组(1.43±0.67,P=0.007),不同TNM分期肿瘤患者的外周血白细胞端粒DNA长度差异无统计学意义。将患者按外周血端粒DNA长度分组,短端粒组患者平均生存期17.149个月(95%CI=14.696~19.602),明显低于长端粒组平均生存期(30.857个月,95%CI=28.515~33.199),差异有统计学意义(P=0.001);Cox比例风险回归分析发现,端粒DNA长度(P=0.041)、肿瘤分期(P=0.007)是患者预后的独立风险因子。结论肺腺癌患者的外周血白细胞端粒DNA长度大于其他非小细胞肺癌;肿瘤的TNM分期、外周血白细胞端粒DNA长度均为非小细胞肺癌患者预后的独立风险因子。Objective To investigate the relationship between telomere DNA length of peripheral leukocytes and tumor type and prognosis in non-small cell lung cancer(NSCLC).Methods From June 2016 to April 2017,the thoracic surgery department of Baoding First Central Hospital,Hebei Province was included in the study.The patients with lung cancer and TNM stage were confirmed by pathological examination,excluding the patients with unknown general records and lost visits.Finally,55 patients were included,including 35 patients in adenocarcinoma group and 20 patients in other non-small cell lung cancer group.The telomere DNA length of peripheral blood leukocytes in patients with non-small cell lung cancer was detected by real-time fluorescence quantitative PCR,and the survival of patients was followed up to analyze the relationship between telomere DNA length of peripheral blood leukocytes and tumor pathological type and prognosis.Results The telomere DNA length(T/s)(1.98±0.69)of adenocarcinoma group was larger than that of other non-small cell lung cancer group(1.43±0.67),P=0.007;there was no significant difference in the telomere DNA length of peripheral blood leukocytes in patients with different TNM stages.According to the length of telomere DNA in peripheral blood,the average survival time of short telomere group was 17.149 months(95%CI=14.696~19.602),which was significantly lower than that of long telomere group(30.857 months,95%CI=28.515~33.199),the difference was statistically significant(P=0.001);Cox proportional risk regression analysis showed that:telomere DNA length(P=0.041),tumor stage(P=0.007)were independent risk factors for prognosis of patients.Conclusion Telomere DNA length of peripheral blood leukocytes in patients with lung adenocarcinoma is longer than that of other non-small cell lung cancer,and TNM stage and telomere DNA length of peripheral blood leukocytes are independent risk factors for prognosis of non-small cell lung cancer.

关 键 词：非小细胞肺癌 端粒DNA长度 肺癌 预后 

分 类 号：R73[医药卫生—肿瘤]