Lgr5和Ki-67蛋白在胃癌组织中的表达研究  

Research of Lgr5 and Ki-67 protein expressions in gastric cancer tissue

作　　者：冯宁[1] 朱瑞萍[2] 程瑶 林峰[1] 薛帆[1] 崔佑刚[1] 张旭[1] 马洋 刘文志[1] FENG Ning;ZHU Ruiping;CHENG Yao;LIN Feng;XUE Fan;CUI Yougang;ZHANG Xu;MA Yang;LIU Wenzhi(Department of General Surgery,Affiliated Zhongshan Hospital of Dalian University,Dalian,Liaoning 116001,P.R.China;Department of Pathology,Affiliated Zhongshan Hospital of Dalian University,Dalian,Liaoning 116001,P.R.China)

机构地区：[1]大连大学附属中山医院普外科,辽宁大连116001 [2]大连大学附属中山医院病理科,辽宁大连116001

出　　处：《中国普外基础与临床杂志》2019年第11期1293-1297,共5页Chinese Journal of Bases and Clinics In General Surgery

基　　金：2016年大连市卫计委课题资助项目（项目编号1611109）

摘　　要：目的检测Lgr5和Ki-67蛋白在胃癌组织及其正常胃黏膜组织中的表达情况并分析其在胃癌发生及发展中的作用。方法采用SABC免疫组织化学方法测定69例胃癌患者的胃癌组织及其相应的正常胃黏膜组织中Lgr5和Ki-67蛋白表达情况并分析其与胃癌患者临床病理特征的关系。结果Lgr5和Ki-67蛋白在胃癌组织中的表达阳性率均较其在正常胃黏膜组织中高[Lgr5蛋白:87.0%(60/69)比16.7%(5/30),χ2=45.81,P<0.001;Ki-67蛋白:79.7%(55/69)比36.7%(11/30),χ2=17.43,P<0.001]。Lgr5及Ki-67蛋白在低-未分化、N1~N3期及幽门螺杆菌(HP)感染阳性胃癌组织中的蛋白表达阳性率明显高于高-中分化、N0期及HP感染阴性患者(P<0.050),Lgr5蛋白表达还与胃癌浸润深度有关(P<0.050),而Ki-67蛋白表达与此无关(P>0.050)。Lgr5及Ki-67蛋白表达与胃癌患者的性别和远处转移均无关(P>0.050)。Lgr5蛋白表达与Ki-67蛋白表达呈明显的正相关关系(rs=0.340,P=0.004)。结论Lgr5蛋白可能参与了肿瘤组织的局部浸润和淋巴结转移过程并与肿瘤恶性程度有关,Ki-67蛋白也可能参与了肿瘤淋巴结转移过程且也与肿瘤恶性程度有关,且此二者在肿瘤发生和发展中可能具有协同作用,同时HP感染可能与Lgr5和Ki-67蛋白协同参与了肿瘤发生、发展过程。Objective To determine the expressions of Lgr5 protein and Ki-67 protein in gastric cancer tissues,and to analyze the possible function in the carcinogenesis and development of gastric cancer.Methods The SABC immunohistochemical method was adopted to examine the expressions of Lgr5 protein and Ki-67 protein in the 69 paraffin slices of gastric cancer from the patients,with the adjacent normal gastric tissue as the control group.The statistical relationship between the expressions of these two kinds of proteins and clinicopathologic features of gastric cancer was examined respectively.Results In the gastric cancer tissue group,the expressions of Lgr5 protein and Ki-67 protein upregulated in comparison to the adjacent normal gastric tissue group[Lgr5 protein:87.0%(60/69)versus 16.7%(5/30),χ2=45.81,P<0.001;Ki-67 protein:79.7%(55/69)versus 36.7%(11/30),χ2=17.43,P<0.001].The expressions of Lgr5 protein and Ki-67 protein all upregulated in the N1–N3 stage groups,lowly differentiated+undifferentiated groups and positive Helicobacter pylori(HP)groups.The expression of Lgr5 protein upregulated in the T3+T4 stage groups in comparison to T1+T2 stage groups,while,no significant relationship was found in the expression of Ki-67 protein and tumor T staging.No significant relationship was found between the gender or metastasis and the expression of these two proteins.There was a positive correlation between the Lgr5 protein expression and the Ki-67 protein expression in the gastric cancer(rs=0.340,P=0.004).Conclusions In the development progress of gastric cancer,the Lgr5 protein might get involved in the mechanism of tumor invasion,lymph nodal metastasis,and low differentiation.Ki-67 protein might get involved in the mechanism of lymph nodal metastasis and low differentiation.The two proteins,together with the HP infection,might play a synergistic role in tumorigenesis and development.

关 键 词：胃癌 Lgr5蛋白 KI-67蛋白 肿瘤干细胞 幽门螺杆菌 

分 类 号：R73[医药卫生—肿瘤]