β3肾上腺素能受体激动剂诱导小鼠脂肪组织棕色化的时效性  

Timeliness of β3 adrenergic receptor agonist-induced browning of white adipose tissues in mice

作　　者：贾如 黄佳琪 魏晓静 胡波 罗肖 JIA Ru;HUANG Jiaqi;WEI Xiaojing;HU Bo;LUO Xiao(Department of Prosthodontics,Key Laboratory of Shaanxi Province for Craniofacial Precision Medicine Research,College of Stomatology,Xi’an Jiaotong University,Xi'an 710004;Department of Physiology and Pathophysiology,Xi’an Jiaotong University School of Medicine,Key Laboratory of Environment and Genes Related to Diseases,Ministry of Education,Xi’an 710061,China)

机构地区：[1]西安交通大学附属口腔医院修复科,陕西省颅颌面精准医学研究重点实验室,西安710004 [2]西安交通大学医学部基础医学院生理学与病理生理学系,教育部环境与疾病相关基因重点实验室,西安710061

出　　处：《中南大学学报（医学版）》2019年第10期1099-1106,共8页Journal of Central South University ：Medical Science

基　　金：国家自然科学基金(81871190);中央高校基本科研业务费专项资金(xjj2016089)~~

摘　　要：目的:研究β3肾上腺素能受体激动剂CL316,243在体诱导小鼠各部位脂肪组织棕色化的时效性,明确其潜在的分子调控机制。方法:以10周龄C57BL/6J小鼠为研究对象,实验组分别腹腔注射CL316,243(1μg/g)1,3及5 d,对照组注射无菌生理盐水溶剂。通过免疫组织化学法和基因表达分析等,观察不同作用时间下小鼠各部位脂肪组织的棕色化改变及产热相关基因及蛋白质的表达水平。结果:与对照组相比,在腹腔注射CL316,243第1天时附睾周围白色脂肪组织(epididymal white adipose tissue,eWAT)的重量就明显减轻,并且随着给药时间的延长,腹股沟皮下白色脂肪组织(inguinal subcutaneous white adipose tissue,sWAT)和eWAT中解偶联蛋白UCP-1 mRNA和蛋白质表达升高;而持续注射5 d能显著抑制小鼠的摄食及体重增加,并诱导小鼠肩胛部棕色脂肪(interscapular brown adipose tissue,iBAT)、sWAT及eWAT中细胞直径的缩小及小脂滴的聚集。结论:β3肾上腺素能受体激动剂CL316,243能在体诱导小鼠脂肪组织的棕色化,并且在不同脂肪组织中表现出不同的时效性特点。Objective:To characterize the timeliness ofβ3 adrenergic receptor agonist CL316,243-induced browning of white adipose tissues in mice.Methods:Male C57BL/6J mice at 10 weeks of age were housed in conventional cages and given sterile saline for the control group or CL316,243(1μg/g)for the experimental group via intraperitoneal injection for 1,3,and 5 days.Food intake and body weight were measured daily.Interscapular brown adipose tissue(iBAT),inguinal subcutaneous white adipose(sWAT)and epididymal white adipose tissue(eWAT)were harvested for histological and gene expression analysis.Results:Compared with the control group,intraperitoneal injection of CL316,243 reduced the weight of eWAT on the first day.Meanwhile,CL316,243 continuously promoted the mRNA and protein expression of uncoupling protein-1(UCP-1)in sWAT and eWAT.Furthermore,CL316,243 injection significantly decreased the food intake and weight gain of the mice,and reduced the diameter of adipocyte and accumulation of small lipid droplets in adipose tissues.Conclusion:CL316,243 can induce the brown-like remodeling in adipose tissues of mice in vivo,which show different time-dependent manners in different adipose tissues.

关 键 词：脂肪组织棕色化 beige细胞 β3肾上腺素能受体激动剂 部位特异性 

分 类 号：R73[医药卫生—肿瘤]