乳粉中维生素检测标准菌接种培养时间研究  

Study on the inoculation and culture time of vitamin test standard bacteria in milk powder

作　　者：张雅伦 张涛 陈晨 刘红冉 张薇 杨岚 周巍 ZHANG Ya-Lun;ZHANG Tao;CHEN Chen;LIU Hong-Ran;ZHANG Wei;YANG Lan;ZHOU Wei(Hebei Food Inspection and Research Institute,Hebei Food Safety Key Laboratory,Shijiazhuang 050071,China)

机构地区：[1]河北省食品检验研究院河北省食品安全重点实验室

出　　处：《食品安全质量检测学报》2019年第19期6607-6610,共4页Journal of Food Safety and Quality

基　　金：科技部“食品安全关键技术研发”重点专项项目(2017FYC1601400);河北省人才工程培养资助项目(A201905002)~~

摘　　要：目的探究乳粉中维生素检测标准菌在接种前的最佳培养时间。方法测定标准菌的不同培养时间下的紫外吸收值,分析得出生长曲线来确定标准菌最佳接种时期。结果泛酸、生物素和维生素B12标准菌最佳接种培养时间为16 h,叶酸标准菌为14 h。接种培养时间远低于现行标准规定时间,且叶酸标准菌活性和活菌数量随时间延长明显下降,其他标准菌也有所减弱。结论通过缩短接种前的培养时间,提高后续奶粉检测的准确性,减少因标准菌接种状态带来的不利影响。Objective To explore the optimal culture time of vitamin test standard bacteria in milk powder before inoculation. Methods Ultraviolet Spectrophotomete absorption values of standard bacteria at different culture time were determined, and growth curves were obtained to determine the optimal inoculation time of standard bacteria. Results The optimal inoculation and culture time of pantothenic acid, biotin and vitamin B12 standard bacteria was 16 h and folic acid standard bacteria was 14 h. The inoculation and culture time was much lower than the current standard, and the activity of folic acid standard bacteria and the number of live bacteria decreased obviously with the extension of time, and other standard bacteria also weakened. Conclusion By shortening the culture time before inoculation, improving the accuracy of subsequent milk powder testing and reducing the adverse effects caused by the inoculation status of standard bacteria.

关 键 词：维生素 标准菌 生长曲线 接种培养时间 

分 类 号：TS2[轻工技术与工程—食品科学与工程]