TGF-β通过Lnc-ATB促进食管癌细胞侵袭转移的机制研究  

Mechanism of TGF-β promoting invasion and metastasis of esophageal cancer cells through Lnc-ATB

作　　者：鲁之中[1] 李军民[1] 胡云芝[2] 李晓辉[3] 李卫[1] 姜富国 李帅 刘学庆[4] Zhi-zhong Lu;Jun-min Li;Yun-zhi Hu;Xiao-hui Li;Wei Li;Fu-guo Jiang;Shuai Li;Xue-qing Liu(Clinical Laboratory,Jiaozuo People’s Hospital,Jiaozuo,Henan 454002,China;Department of Pediatrics,Jiaozuo People’s Hospital,Jiaozuo,Henan 454002,China;Department of Surgical Oncology,Jiaozuo People’s Hospital,Jiaozuo,Henan 454002,China;School of Public Health and Management,Chongqing Medical University,Chongqing 400016,China)

机构地区：[1]焦作市人民医院检验科,河南焦作454002 [2]焦作市人民医院儿科,河南焦作454002 [3]焦作市人民医院肿瘤外科,河南焦作454002 [4]重庆医科大学公共卫生与管理学院,重庆400016

出　　处：《中国现代医学杂志》2019年第22期7-11,共5页China Journal of Modern Medicine

摘　　要：目的探讨转化生长因子-β(TGF-β)对食管癌细胞侵袭转移的影响及其作用机制。方法将食管癌细胞加入TGF-β后,通过实时荧光定量聚合酶链反应检测食管癌细胞EC9706中被TGF-β活化的长链非编码RNA(Lnc-ATB)的表达情况,通过Transwell实验检测TGF-β对EC9706细胞侵袭转移的影响;沉默EC9706细胞中Lnc-ATB的表达,给予或不给予TGF-β刺激,利用Transwell实验检测EC9706细胞侵袭转移的情况。结果Lnc-ATB-siRNA组Lnc-ATB较Lnc-ATB-NC组低(P<0.05),Lnc-ATB siRNA可有效沉默EC9706细胞中Lnc-ATB的表达。TGF-β组TGF-β处理后EC9706细胞的侵袭和转移细胞数均高于对照组(P<0.05)。TGF-β+Lnc-ATB-NC组TGF-β通过Lnc-ATB上调EC9706细胞侵袭及转移能力高于其他组(P<0.05);空白组细胞侵袭能力高于TGF-β+Lnc-ATB-siRNA组和Lnc-ATB-siRNA组(P<0.05)。结论食管癌中TGF-β通过上调Lnc-ATB的表达,促进细胞的侵袭及转移。Objective To investigate the function and mechanism on invasion and metastasis of TGF-βin esophageal carcinoma.Methods EC9706 cells were treated with TGF-β,and then Lnc-ATB expression in EC9706 cells was detected by quantitative real time PCR(qRT-PCR);the invasion and metastasis of EC9706 cells were analyzed by Transwell;after silencing the expression of Lnc-ATB in EC9706 cells,treated with TGF-βor not,the invasion and metastasis of EC9706 cells was analyzed by Transwell.Results The expression of Lnc-ATB was higher in Lnc-ATB-siRNA group compared with Lnc-ATB-NC group(P<0.05),and Lnc-ATB siRNA can effectively silence the expression of Lnc-ATB in EC9706 cells;the invasion and metastasis of EC9706 cells was significantly upregulated after treated with TGF-β(P<0.05);the ability of invasion and metastasis promoted by Lnc-ATB after treated with TGF-βof EC9706 cells in TGF-β+Lnc-ATB-NC group was higher than in other groups(P<0.05);the ability of invasion in control group was higher than in TGF-β+Lnc-ATB-siRNA group and Lnc-ATB-siRNA group(P<0.05).Conclusions TGF-βpromote the invasion and metastasis of cells via up-regulating the expression of Lnc-ATB in esophagus cancer.

关 键 词：食管肿瘤 肿瘤浸润 肿瘤转移 

分 类 号：R735.1[医药卫生—肿瘤]