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作 者:郭静[1] 王浩然 沈周媛 张彤[1] 袁秀荣[1] 丁越[1] GUO Jing;WANG Hao ran;SHEN Zhou yuan;ZHANG Tong;YUAN Xiu rong;DING Yue(Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China;Xiaqiao Community Health Service Center of Xuzhou,Xuzhou 221011,China)
机构地区:[1]上海中医药大学,上海201203 [2]徐州市夏桥社区卫生服务中心,江苏徐州221011
出 处:《中成药》2019年第11期2688-2694,共7页Chinese Traditional Patent Medicine
基 金:国家自然基金项目(81872781);上海中医药大学杏林青年学者(2017);上海中医药大学大学生科创项目第十一期科创项目(2018140);上海优秀学术带头人计划(18XD1403700)
摘 要:目的研究3种竹叶中有效成分含有量与其提取物抗氧化活性差异的相关性及其抗氧化活性基础物质。方法采用HPLC法分别测定了7批淡竹叶、10批苦竹叶、9批竹叶中7种成分的含有量;DPPH法、ABTS法及FRAP法测定其抗氧化活性。以清除DPPH自由基的能力评价3种竹叶提取物的抗氧化活性,并利用多元相关与回归分析方法探讨各成分含有量与提取物活性的关系。结果淡竹叶的抗氧化活性最弱,且与苦竹叶、竹叶比较均具有显著性差异(P<0.05)。芹菜素、异荭草苷、荭草苷含有量分别与淡竹叶、苦竹叶、竹叶抗氧化活性呈显著性正相关(P<0.05)。回归分析结果与相关性分析结果基本一致。结论芹菜素、异荭草苷、荭草苷分别是淡竹叶、苦竹叶、竹叶中的主要抗氧化有效成分。AIM To study the functional antioxidant compounds in three kinds of bamboo leaves and the correlations between the content of effective components and antioxidant activities of the extracts.METHODS The contents of seven components in seven batches of Lophatherum gracile Brongn.,ten batches of Pleioblastus amarus(Keng) Keng f.and nine batches of Phyllostachys nigra(Lodd.) Munro var:henonis(Mitf.) Stapf ex Rendle leaves were determined by HPLC,and DPPH,ABTS,FRAP assay were used to evaluate their antioxidant capacity.The antioxidant activities of extracts were evaluated by the capability of scavenging DPPH free radical, and the relationships between the contents of compounds and the activities of extracts were explored by multiple correlation and regression analysis.RESULTS The antioxidant activities of L. gracile leaves were the weakest,and they were significantly different from P. amarus and P. nigra(P<0.05).The contents of apigenin,isoorientin,and orientin were significantly positively correlated with antioxidant activities of L. gracile,P. Amarus and P. nigra leaves(P<0.05), respectively.Stepwise regression analysis results were the same as bivariate correlation analysis results.CONCLUSION Apigenin,isoorientin,and orientin are the main effective components in L. gracile,P. Amarus, and P. nigra leaves, respectively.
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