HPLC法同时测定不同品种红花中4种黄酮类  

Simultaneous determination of four flavonoids in Carthamus tinotorius of different varieties by HPLC

作　　者：马梦雪 吴士筠[1] 李刚[1] 覃瑞[1] 董翔 刘虹[1] MA Meng xue;WU Shi jun;LI Gang;QIN Rui;DONG Xiang;LIU Hong(Hubei Provincial Key Laboratory for Protection and Application of Special Plant Germplasm in Wuling Area of China,College of Life Sciences,South Central University for Nationalities,Wuhan 430074,China)

机构地区：[1]中南民族大学生命科学学院武陵山区特色资源植物种质保护与利用湖北重点实验室

出　　处：《中成药》2019年第11期2694-2700,共7页Chinese Traditional Patent Medicine

基　　金：国家科学技术部科技基础性工作专项重点项目资助(2014FY110100);湖北省科技条件平台建设专项(2017BEC014);湖北重点实验室建设基金(2018BFC360)

摘　　要：目的建立HPLC法同时测定不同红花Carthamus tinctorius L.(Safflower)中羟基红花黄色素A、芦丁、木犀草素、槲皮素的含有量。方法红花60%甲醇提取物的分析采用Agilent ZORBAX SB-C18色谱柱(4.6 mm×150 mm,5μm);流动相乙腈-0.5%乙酸,梯度洗脱;体积流量0.5 mL/min;柱温25℃;检测波长300 nm。结果羟基红花黄色素A、芦丁、木犀草素、槲皮素分别在17.36~282(R2=0.999 7)、1.68~181.84(R2=0.999 5)、0.52~8.71(R2=0.999 5)、5.17~94.68(R2=0.999 4)μg/mL的范围内线性关系良好,平均加样回收率96.83%~98.39%,RSD 1.13%~2.36%。结论该方法准确稳定,重复性好,可用于红花的质量控制。AIM To establish an HPLC method for the simultaneous content determination of hydroxysafflor yellow A,rutin,luteolin and quercetin in Carthamus tinotorius L.(Safflower).of different varieties.METHODS The analysis of 60%methanol extract of C.tinotorius was performed on a 25℃thermostatic Agilent ZORBAX SB-C18 column(4.6 mm×150 mm,5μm),with the mobile phase comprising of acetonitrile-0.5%acetic acid flowing at 0.4 mL/min in a gradient elution manner,and the detection wavelength was set at 300 nm.RESULTS Hydroxysafflor yellow A,rutin,luteolin and quercetin showed good linear relationships within the ranges of 17.36-282(R2=0.9997)、1.68-181.84(R2=0.9995)、0.52-8.71(R2=0.9995)、5.17-94.68(R2=0.9994)μg/mL,whose average recoveries were 96.83%-98.39%with the RSDs of 1.13%-2.36%,respectively.CONCLUSION This accurate,stable and reproducible method can be used for the quality control of C.tinotorius.

关 键 词：红花 羟基红花黄色素A 芦丁 木犀草素 槲皮素 HPLC 

分 类 号：R284[医药卫生—中药学]