miRNA-375通过MYC/EMT对NSCLC细胞侵袭和迁移影响  

MIRNA-375 AFFECTS THE INVASION AND MIGRATION OF NON-SMALL CELL LUNG CANCER CELLS VIA MYC/EPITHE-LIAL-MESENCHYMAL TRANSITION

作　　者：孟海宁 高钿[2] 仵俊宇 黄巧 徐晟伟[3] 沈若武[1] MENG Haining;GAO Tian;WU Junyu;HUANG Qiao;XU Shengwei;SHEN Ruowu(Department of Special Medicine,School of Basic Medicine,Qingdao University,Qingdao 266071,China;不详)

机构地区：[1]青岛大学基础医学院特种医学系,山东青岛266071 [2]青岛市市立医院产三科 [3]青岛市第三人民医院呼吸科

出　　处：《青岛大学学报（医学版）》2019年第6期639-642,647,共5页Journal of Qingdao University(Medical Sciences)

基　　金：山东省高等学校科技计划项目(L16LK04)

摘　　要：目的探究miRNA-375(miR-375)通过MYC/上皮间质转化(EMT)对非小细胞肺癌(NSCLC)侵袭和迁移的影响。方法将未做任何处理的NSCLC细胞A549作为空白组,转染空载慢病毒的A549细胞作为对照组,转染表达miR-375慢病毒的A549细胞作为实验组。采用实时荧光定量PCR(qRT-PCR)技术检测miR-375在NSCLC组织和癌旁组织中的表达量,以及miR-375在正常肺组织细胞(BEAS-2B细胞)和A549细胞中的表达量;通过Transwell实验检测3组细胞的侵袭、迁移能力;采用Western blot检测3组细胞中MYC蛋白和EMT相关蛋白(E-钙黏蛋白、波形蛋白)的表达量。结果miR-375在NSCLC组织中的表达量显著高于癌旁组织(t=16.88,P<0.05),在A549细胞中的表达量显著高于BEAS-2B细胞(t=8.51,P<0.05)。过表达miR-375后,实验组与空白组和对照组相比,NSCLC细胞的侵袭(F=29.55,P<0.05)、迁移(F=90.21,P<0.05)能力增强,MYC蛋白表达增加(F=37.15,P<0.05),E-钙黏蛋白表达减少(F=47.25,P<0.05),波形蛋白表达增加(F=77.64,P<0.05)。结论上调miR-375可以通过MYC/EMT影响NSCLC的侵袭和迁移能力。Objective To investigate the effect of miRNA-375(miR-375)on the invasion and migration of non-small cell lung cancer(NSCLC)via MYC/epithelial-mesenchymal transition(EMT).Methods NSCLC A549 cells without any treatment were established as blank group,A549 cells transfected with empty vector lentivirus were established as control group,and A549 cells transfected with miR-375 lentiviral vector were established as experimental group.Quantitative real-time PCR was used to measure the expression of miR-375 in NSCLC tissue and adjacent tissue,as well as in BEAS-2B cells and A549 cells.Transwell assay was used to measure the invasion and migration abilities of A549 cells,and Western blot was used to measure the expression of MYC protein and EMT-related proteins(E-cadherin and vimentin)in the three groups of cells.Results The NSCLC tissue had significantly higher expression of miR-375 than the adjacent tissue(t=16.88,P<0.05),and A549 cells had significantly higher expression of miR-375 than BEAS-2B cells(t=8.51,P<0.05).After the overexpression of miR-375,compared with the blank group and the control group,the experimental group had significant increases in the abilities of invasion(F=29.55,P<0.05)and migration(F=90.21,P<0.05),a significant increase in the protein expression of MYC(F=37.15,P<0.05),a significant reduction in the expression of E-cadherin(F=47.25,P<0.05),and a significant increase in the expression of vimentin(F=77.64,P<0.05).Conclusion Upregulation of miR-375 can promote the invasion and migration of NSCLC through MYC/EMT.

关 键 词：微RNAS 癌 非小细胞肺 基因 myc 上皮-间质转化 细胞运动 

分 类 号：R734.2[医药卫生—肿瘤] R342.2[医药卫生—临床医学]