PTPN14过表达对子宫内膜癌HEC-1A细胞增殖和凋亡影响  

EFFECT OF THE OVEREXPRESSION OF PROTEIN TYROSINE PHOSPHATASE, NON-RECEPTOR TYPE 14 ON THE PROLI-FERATION AND APOPTOSIS OF ENDOMETRIAL CARCINOMA HEC-1A CELLS

作　　者：袁雪莲[1] 乐珍 刘国炳 YUAN Xuelian;LE Zhen;LIU Guobing(Department of Obstetrics and Gynecology,The First People’s Hospital of Zhengzhou,Zhengzhou 450018,China;不详)

机构地区：[1]郑州市第一人民医院产科,河南郑州450018 [2]南方医科大学南方医院妇产科 [3]南方医科大学病理学系

出　　处：《青岛大学学报（医学版）》2019年第6期705-708,713,共5页Journal of Qingdao University(Medical Sciences)

基　　金：广东省科技计划项目(2014A020212628)

摘　　要：目的探讨非受体蛋白质酪氨酸磷酸酶14(PTPN14)过表达对子宫内膜癌HEC-1A细胞增殖和凋亡的影响及其机制。方法将体外培养的HEC-1A细胞分为A组(未转染)、B组(转染阴性对照质粒)和C组(转染PTPN14过表达质粒)。采用蛋白质印迹(Western blot)法检测PTPN14蛋白和Yes相关蛋白(YAP蛋白)的表达,定量实时聚合酶链反应(qRT-PCR)检测PTPN 14 mRNA和YAP mRNA的表达;细胞计数(CCK-8)法检测细胞增殖;流式细胞仪检测细胞凋亡。结果与A组比较,C组细胞中PTPN14蛋白和mRNA的表达水平以及细胞凋亡率均显著升高,细胞增殖活力以及YAP蛋白和mRNA的表达水平均显著降低,差异均具有统计学意义(t=5.752~37.554,P<0.01);而B组和C组间差异均无统计学意义(P>0.05)。结论过表达PTPN14能够抑制子宫内膜癌细胞增殖,诱导细胞凋亡,其作用机制可能与下调YAP蛋白表达有关。Objective To investigate the effect of the overexpression of protein tyrosine phosphatase,non-receptor type 14(PTPN14)on the proliferation and apoptosis of endometrial carcinoma HEC-1A cells and related mechanism.Methods HEC-1A cells cultured in vitro were divided into group A(untransfected),group B(transfected with negative control plasmid),and group C(transfected with PTPN14-overexpression plasmid).Western blot was used to measure the protein expression of PTPN14 and Yes-related protein(YAP),and qRT-PCR was used to measure the mRNA expression of PTPN14 and YAP.CCK-8 assay was used to measure cell proliferation,and flow cytometry was used to detect cell apoptosis.Results Compared with group A,group C had significant increases in the protein and mRNA expression of PTPN14 and apoptosis rate of cells,as well as significant reductions in cell proliferation activity and the protein and mRNA expression of YAP(t=5.752-37.554,P<0.01),and there were no significant differences between group B and group C(P>0.05).Conclusion Overexpression of PTPN14 can inhibit the proliferation of endometrial carcinoma cells and induce apoptosis,possibly by downregulating the expression of YAP.

关 键 词：子宫内膜肿瘤 蛋白质酪氨酸磷酸酶 非受体14型 细胞增殖 细胞凋亡 YAP蛋白 

分 类 号：R737.33[医药卫生—肿瘤]