双纸片增效法与协同法在131株致病菌检测β-内酰胺酶中的应用  

Application of Double-Disc Efficacy and Synergy Methods in Detection of beta-Lactamase in 131 Strains of Pathogenic Bacteria

作　　者：李漫[1] LI Man(Department of Clinical Laboratory,The Ninth People's Hospital,Shanghai Jiao Tong University School of Medicine,Shanghai 201900)

机构地区：[1]上海交通大学医学院附属第九人民医院检验科,上海201900

出　　处：《大医生》2019年第10期44-45,共2页Doctor

摘　　要：目的比较双纸片增效法与双纸片协同法在非发酵菌β-内酰胺酶检测中的应用,比较两种检测方法的阳性率等指标。方法以头孢他定为底物,乙二胺四乙酸二钠(EDTA.2Na)为酶抑制剂,运用双纸片增效法和双纸片协同法共同检测131株非发酵菌是否产生β-内酰胺酶。结果131株中有38株双纸片增效法阳性,有120株双纸片协同法阳性。结论双纸片协同法较双纸片增效法结果直观,影响因素少,且阳性率高。Objective To compare the application of double-disc efficacy method and double-disc synergy method in the detection of beta-lactamase in non-fermenting bacteria,and to compare the positive rates of the two methods.Methods Using ceftazidime as substrate and EDTA.2Na as enzyme inhibitor,131 strains of non-fermenting bacteria were tested forβ-Lactamase production by double-disc efficacy method and double-disc synergy method.Results Among 131 strains,38 were positive by double-disc efficacy method and 82 were positive by double-disc synergy method.Conclusion The results of double-disc synergy method are more intuitive than that of double-disc efficacy method,with fewer influencing factors and higher positive rate.

关 键 词：非发酵菌 Β-内酰胺酶 双纸片增效法 双纸片协同法 

分 类 号：G63[文化科学—教育学]