Effects of Leaf Extract of Phyllanthus reticulatus from Guangxi on Human Gastric Cancer SGC-7901 Cells in vitro  

作　　者：Yunli TANG Jiangcun WEI Huaien WU Chenyan LIANG Zuowen ZHENG Qiuheng RUAN Yuanzhi WU 

机构地区：[1]Guangxi University of Chinese Medicine,Nanning 530001,China [2]International Zhuang Medical Hospital Affiliated to Guangxi University of Chinese Medicine,Nanning 530201,China

出　　处：《Medicinal Plant》2019年第5期45-49,共5页药用植物：英文版

基　　金：Supported by Natural Science Research Project of Guangxi University of Chinese Medicine in 2015"Study on Anti-tumor Effect of Phyllanthus reticulatus Leaf Extract"(p15028);Independent Research Program of Guangxi Key Laboratory of Traditional Chinese Medicine Pharmacology in 2015"Study on Phyllanthus reticulatus Leaf 7404 Nude Mice Xenografts";Young Teachers Enhancement Project of Guangxi Department of Education in 2018"Study on the Mechanism of Zhuang Medicine Phyllanthus reticulatus Leaf against Liver Cancer"(2018KY0300);2019-2021 Guangxi First-class Discipline Construction Open Project Fund for Young Scholars of Guangxi University of Chinese Medicine"Anti-hepatocarcinoma Mechanism of Autophagy Induced by Ethyl Acetate in Phyllanthus reticulatus Leaf Based on PI3K/AKT and STAT3/m TOR Signaling Pathway"(2019XK089)

摘　　要：[Objectives]To observe the effect of drug-containing serum from different extracts of Phyllanthus reticulatus leaf on the proliferation of human gastric cancer SGC-7901 cells and the effect of in vivo on the life extension rate of mice with H22 ascites tumor,and to investigate the effects of chemical components such as Corilagin,ethyl gallate,ethyl brevifolincarboxylate,and gallic acid on the proliferation of human gastric cancer SGC-7901 cells.[Methods]MTT method was used to observe the inhibitory effect of drug-containing serum of different extracts of P.reticulatus leaf on human gastric cancer SGC-7901 cells in vitro to determine its active site.The active site was used as the research object to establish a Kunming mouse ascites tumor model,to investigate the effect on the life extension rate of mice with H22 ascites tumor,to further separate the monomer components from the effective fraction and investigate its effect on human gastric cancer SGC-7901 cells.[Results]Compared with the 10%blank serum control group,10%drug-containing serum of ethyl acetate and n-butanol in P.reticulatus leaf had significant inhibitory effect on the proliferation of human gastric cancer SGC-7901 cells,and the inhibition rate was 34.99%and 28.68%,respectively(P<0.05).Compared with the blank group,the survival time of the high dose group of ethyl acetate in P.reticulatus leaf was significant(P<0.05).Gallic acid,Corilagin,and Brevifolincarboxylic acid ethyl ester had a significant inhibitory effect on the proliferation of human gastric cancer SGC-7901 cells,with IC 50 of 26.52,70.45,and 158.86μg/mL,respectively.Ethyl gallate had no inhibitory effect on the proliferation of human gastric cancer SGC-7901 cells,and its IC 50 was 251.96μg/mL.[Conclusions]The drug-containing serum of ethyl acetate and n-butanol extract of P.reticulatus can inhibit the proliferation of human gastric cancer SGC-7901 cells.Ethyl acetate of P.reticulatus leaves can increase the life extension rate of mice with H22 ascites tumor.Gallic acid,Corilagin,and Bre[Objectives] To observe the effect of drug-containing serum from different extracts of Phyllanthus reticulatus leaf on the proliferation of human gastric cancer SGC-7901 cells and the effect of in vivo on the life extension rate of mice with H22 ascites tumor, and to investigate the effects of chemical components such as Corilagin, ethyl gallate, ethyl brevifolincarboxylate, and gallic acid on the proliferation of human gastric cancer SGC-7901 cells. [Methods] MTT method was used to observe the inhibitory effect of drug-containing serum of different extracts of P. reticulatus leaf on human gastric cancer SGC-7901 cells in vitro to determine its active site. The active site was used as the research object to establish a Kunming mouse ascites tumor model, to investigate the effect on the life extension rate of mice with H22 ascites tumor, to further separate the monomer components from the effective fraction and investigate its effect on human gastric cancer SGC-7901 cells. [Results] Compared with the 10% blank serum control group, 10% drug-containing serum of ethyl acetate and n-butanol in P. reticulatus leaf had significant inhibitory effect on the proliferation of human gastric cancer SGC-7901 cells, and the inhibition rate was 34.99% and 28.68%, respectively（P<0.05）. Compared with the blank group, the survival time of the high dose group of ethyl acetate in P. reticulatus leaf was significant（P<0.05）. Gallic acid, Corilagin, and Brevifolincarboxylic acid ethyl ester had a significant inhibitory effect on the proliferation of human gastric cancer SGC-7901 cells, with IC50 of 26.52, 70.45, and 158.86 μg/mL, respectively. Ethyl gallate had no inhibitory effect on the proliferation of human gastric cancer SGC-7901 cells, and its IC50 was 251.96 μg/mL. [Conclusions] The drug-containing serum of ethyl acetate and n-butanol extract of P. reticulatus can inhibit the proliferation of human gastric cancer SGC-7901 cells. Ethyl acetate of P. reticulatus leaves can increase the life extension rate of mice with H2

关 键 词：PHYLLANTHUS reticulatus SGC-7901 cells SERUM PHARMACOLOGY ASCITES tumor 

分 类 号：R73[医药卫生—肿瘤]