不同形式蛋氨酸对黄鳝生长、血清生化、血清游离氨基酸含量及肌肉品质的影响  

EFFECTS OF DIETARY METHIONINE ON GROWTH, SERUM BIOCHEMICAL INDEXES, SERUM FREE AMINO ACID AND MUSCLE TEXTURE OF RICE FIELD EEL(MONOPTERUS ALBUS)

作　　者：胡亚军[1,2] 胡毅 石勇[1,2] 戴振炎 钟蕾[1,2] HU Ya-Jun;HU Yi;SHI Yong;DAI Zhen-Yan;ZHONG Lei(College of Animal Science and Technology,Hunan Agriculture University,Changsha 410128,China;Collaborative Innovation Center for Efficient and Health Production of Fisheries in Hunan Province,Changde 415000,China)

机构地区：[1]湖南农业大学湖南省特色水产资源利用工程技术研究中心,长沙410128 [2]水产高效健康生产湖南省协同创新中心,常德415000

出　　处：《水生生物学报》2019年第6期1155-1163,共9页Acta Hydrobiologica Sinica

基　　金：国家自然科学基金面上项目(31572626);2017年湖南省研究生科研创新项目(CX2017B367)资助~~

摘　　要：以初始体重(30.00±0.45)g的黄鳝(Monopterus albus)为研究对象,以低鱼粉饲料为对照组,在低鱼粉饲料中分别添加蛋氨酸有效含量为2 g/kg的晶体蛋氨酸(C-Met)、包膜蛋氨酸(E-Met)、蛋氨酸羟基类似物钙盐(MHA-Ca)、蛋氨酸羟基类似物(MHA),研究其在低鱼粉黄鳝饲料中的利用效果,共5个处理组,养殖试验持续10周。结果表明:(1)在低鱼粉饲料中添加蛋氨酸均有提高黄鳝增重率和蛋白质效率比、降低饲料系数的趋势,其中MHA-Ca和MHA组与对照组差异显著(P<0.05)。(2)在低鱼粉饲料中添加蛋氨酸均显著提高了肠道胰蛋白酶活力、血清胆汁酸、总胆固醇、总蛋白、尿素氮和血氨含量、肌肉黏附性与胶黏性(P<0.05);添加MHA-Ca和MHA显著提高了肠道淀粉酶活力、血清葡萄糖和高密度脂蛋白胆固醇含量、肝脏谷草转氨酶活力、全鱼粗蛋白含量、肌肉硬度、弹性和咀嚼性(P<0.05),显著降低了黄鳝肝体比与血清谷草转氨酶活力(P<0.05)。(3)对照组在摄食6h出现蛋氨酸吸收峰值,C-Met与MHA-Ca组均在摄食后9h出现蛋氨酸吸收峰值,E-Met组在摄食后12h出现蛋氨酸吸收峰值,MHA组分别在摄食后3h和9h出现蛋氨酸峰值。结果表明,在低鱼粉饲料中添加蛋氨酸均能提高黄鳝生长和氨基酸代谢,且MHA-Ca与MHA效果较佳。To investigate the effects of different forms of dietary methionine on rice field eel(Monopterus albus)[initial weight(30±0.45)g],Crystalline methionine(C-Met),encapsulated methionine(E-Met),methionine hydroxyl analog calcium salt(MHA-Ca)and methionine hydroxy analogue(MHA),whose effective Met content was 2 g/kg,were added in basal diet to form five experimental diets for a 10-week trial.Results showed that methionine in all forms improved weight gain rate and protein efficiency ratio and decreased feed conversion rate of rice field eels with significant change in groups of MHA-Ca and MHA compared with control group(P<0.05).Methionine addition significantly improved activity of trypsin,the contents of serum bile acid,total cholesterol,total protein,urea nitrogen and ammonia,muscle gumminess and adhesiveness(P<0.05).MHA-Ca and MHA addition improved the contents of serum glucose and HDL,activity of intestinal amylase,hepatosomatic GOT,crude protein of whole fish,muscle hardness,springiness and chewiness significantly(P<0.05),but reduced hepatosomatic index and serum glutamic oxalacetic transaminase activity significantly(P<0.05).The peak level of serum methionine appeared at 6h in control group after feeding,but at 9h in C-Met and MHA-Ca groups,at 12h in E-Met group,and at 3h and 9h in MHA group.These results revealed that methionine addition improved growth performance of rice field eels with better effects for MHA-Ca and MHA.

关 键 词：黄鳝 晶体蛋氨酸 包膜蛋氨酸 蛋氨酸羟基类似物钙盐 蛋氨酸羟基类似物 生长 氨基酸代谢 

分 类 号：S965.1[农业科学—水产养殖]