CD97异构体在结肠癌组织中的表达及其临床意义  

Expressions of CD97 isoforms in colon cancer tissues and their clinical significances

作　　者：彭湃 胡超华[1] 韩运涛[1] 徐元兵 沈浩元[1] 余幼林 周宏众[2] Peng Pai;Hu Chaohua;Han Yuntao;Xu Yuanbing;Shen Haoyuan;Yu Youlin;Zhou Hongzhong(Department of General Surgery,Xiaogan Hospital Affiliated to Wuhan University of Science and Technology,Xiaogan Central Hospital,Xiaogan 432000,China;Department of General Surgery,the First Affiliated Hospital of Wenzhou Medical University,Wenzhou 325000,China)

机构地区：[1]武汉科技大学附属孝感医院孝感市中心医院普外科,432000 [2]温州医科大学附属第一医院普外科,325000

出　　处：《肿瘤研究与临床》2019年第10期662-665,共4页Cancer Research and Clinic

基　　金：温州市科技局项目(Y20090068)。

摘　　要：目的研究CD97各异构体mRNA在结肠癌中的表达水平及其临床意义。方法收集2013年12月至2014年5月温州医科大学附属第一医院结肠癌患者50例,以及人结肠癌细胞株SW480和SW620。采用实时荧光定量聚合酶链反应检测CD97人类表皮生长因子(EGF)(1,2,5)、CD97EGF(1,2,3,5)、CD97EGF(1,2,3,4,5)mRNA在结肠癌、癌旁组织及正常结肠组织、SW480和SW620细胞中的表达情况,分析CD97EGF(1,2,5)mRNA表达与临床病理因素之间的关系。结果CD97异构体CD97EGF(1,2,5)、CD97EGF(1,2,3,5)、CD97EGF(1,2,3,4,5)mRNA相对表达量在癌组织中高,而在癌旁组织和正常组织少量表达,差异具有统计学意义(0.71±0.20比0.40±0.09比0.35±0.07,F=107.642,P<0.01;0.45±0.11比0.26±0.05比0.27±0.06,F=94.231,P<0.01;0.41±0.10比0.21±0.05比0.19±0.03,F=165.672,P<0.01)。不同肿瘤浸润深度(T1~T2和T3~T4)和临床分期(Ⅰ~Ⅱ和Ⅲ~Ⅳ)结肠癌患者CD97EGF(1,2,5)mRNA表达水平比较,差异均具有统计学意义(t=-2.582和t=-5.062,P=0.013和P<0.01)。在SW620细胞中CD97EGF(1,2,5)mRNA表达量高于SW480细胞。结论CD97异构体在结肠癌组织中高表达,CD97EGF(1,2,5)可能在结肠癌发展和浸润过程中起着重要作用。CD97异构体可能成为在结肠癌治疗的一个新标志物。Objective To study the mRNA expressions of various CD97 isoforms in colorectal carcinoma tissues and their clinical significances.Methods A total of 50 colon cancer patients in the First Affiliated Hospital of Wenzhou Medical University from December 2013 to May 2014 and human colon cancer cell lines SW480 and SW620 were enrolled.The real-time fluorescence quantitative polymerase chain reaction(qRT-PCR)was used to detect the mRNA expressions of CD97 human epidermal growth factor(EGF)(1,2,5),CD97EGF(1,2,3,5)and CD97EGF(1,2,3,4,5)in colon cancer tissues,adjacent tissues,normal colon tissues,SW480 cells and SW620 cells.The relationship between the mRNA expression of CD97EGF(1,2,5)and the clinicopathological factors was analyzed.Results Compared with those low expressions in adjacent tissues and normal tissues,the mRNA expressions of CD97 isoforms CD97EGF(1,2,5),CD97EGF(1,2,3,5)and CD97EGF(1,2,3,4,5)in cancer tissues were highest,and the differences were statistically significant(0.71±0.20 vs.0.40±0.09 vs.0.35±0.07,F=107.642,P<0.01;0.45±0.11 vs.0.26±0.05 vs.0.27±0.06,F=94.231,P<0.01;0.41±0.10 vs.0.21±0.05 vs.0.19±0.03,F=165.672,P<0.01).In addition,the mRNA expression of CD97EGF(1,2,5)in colon cancer patients was associated with tumor infiltration depth(T1-T2 and T3-T4),clinical stages(Ⅰ-ⅡandⅢ-Ⅳ),and the differences were statistically significant(t=-2.582,P=0.013;t=-5.062,P<0.01).The mRNA expression of CD97EGF(1,2,5)in SW620 cells was higher than that in SW480 cells.Conclusions CD97 isoforms are highly expressed in colon cancer tissues,and CD97EGF(1,2,5)may play an important role in the development and invasion of colon cancer.The CD97 isoforms may be new markers in the treatment of colon cancer.

关 键 词：结肠肿瘤 异构体 CD97 逆转录聚合酶链反应 病理学 临床 

分 类 号：R73[医药卫生—肿瘤]