棉花GR基因家族的全基因组鉴定及分析  

Genome-Wide Identification and Analysis of GR Gene Family in Cotton

作　　者：张传义 许艳超 蔡小彦[1] 王星星[1] 侯宇清 王玉红[1] 王坤波[1] 刘方[1] 周忠丽[1] Zhang Chuanyi;Xu Yanchao;Cai Xiaoyan;Wang Xingxing;Hou Yuqing;Wang Yuhong;Wang Kunbo;Liu Fang;Zhou Zhongli(State Key Laboratory of Cotton Biology/Institute of Cotton Research of Chinese Academy of Agricultural Sciences,Anyang,Henan 455000,China)

机构地区：[1]棉花生物学国家重点实验室/中国农业科学院棉花研究所

出　　处：《棉花学报》2019年第6期482-492,共11页Cotton Science

基　　金：国家重点研发计划(2017YFD0101601,2016YFD0100203)

摘　　要：【目的】谷胱甘肽还原酶基因(Glutathione reductase gene,GR)家族参与植物生长发育和非生物胁迫响应等生物进程,但其在棉花中的特性及功能尚不清楚。本研究通过在异源四倍体陆地棉(Gossypium hirsutum)、海岛棉(G.barbadense)及其可能的二倍体祖先种亚洲棉(G.arboreum)和雷蒙德氏棉(G.raimondii)中对GR基因家族全基因组鉴定与特性分析,分析棉种分化及异源四倍体棉花形成过程中GR基因的进化历程,探讨其在非生物胁迫响应中的作用,为后续相关研究提供理论基础。【方法】利用生物信息学方法鉴定陆地棉、海岛棉、雷蒙德氏棉、亚洲棉的GR基因家族成员;解析GR基因家族成员的理化性质、序列特征、染色体位置、系统发育及表达模式。【结果】共鉴定到18个GR基因家族成员,陆地棉、海岛棉、雷蒙德氏棉和亚洲棉中GR基因数目分别为6、6、3和3个。系统发育分析发现GR基因分为2个亚组,同一亚组基因具有相似的外显子数目和基因结构。对同源基因的非同义突变率(Ka)及同义突变率(Ks)分析发现Ka/Ks值均小于1,表明在进化过程中GR基因经历了较强的纯化选择作用。陆地棉GR基因的表达模式分析表明,所有的GR基因均积极响应胁迫环境,但在不同的非生物胁迫下,基因的表达模式有明显差别。【结论】本研究探讨了GR基因家族在亚洲棉、雷蒙德氏棉、海岛棉和陆地棉基因组中的进化及功能,可为棉花GR基因后续研究提供理论基础。[Objective]Glutathione reductase(GR)gene family is involved in biological processes such as plant growth and abiotic stress response,but its characteristics and functions in cotton have not been known yet.This study aims to explore the role of GR genes in cotton genome evolution and abiotic stress response through the whole genome identification and characterization of GR genes,thus providing a theoretical basis for future studies on the roles of the GR genes in enhancing abiotic stress tolerance in cotton.[Method]The GR genes in Gossypium hirsutum,G.barbadense,G.raimondii and G.arboreum were all identified using bioinformatics software.The physicochemical properties,sequence characteristics,chromosomal location,phylogeny and expression patterns were analyzed.[Result]A total of 18 GR genes were identified.The number of GR genes in G.hirsutum,G.barbadense,G.raimondii and G.arboreum was 6,6,3 and 3,respectively.Phylogenetic analysis revealed that GR genes were divided into two sub-groups.The genes in the same subgroup exhibited similar gene structure in relation to exon-intron ratios.The ratios of the non-synonymous mutations(Ka)and homologous mutations(Ks)were all less than 1,indicating that the GR genes underwent strong purification selection during their evolution process.The analysis of the expression patterns of GR genes in upland cotton indicated that all the GR genes responded actively to the stress environment;but under different abiotic stresses,the gene expression patterns were significantly different.[Conclusion]The study explored the evolution and function of the GR gene family in the four cotton genomes,providing a theoretical basis for future studies of cotton GR genes.

关 键 词：棉花 GR基因 生物信息学 系统发育分析 表达模式分析 

分 类 号：R73[医药卫生—肿瘤]