4种银耳属多糖的理化特征、微观结构及其抗氧化和抗炎症作用研究  

Physicochemical Properties,Microstructure,and Antioxidant and Anti-inflammation Effects of Four Major Polysaccharides from Tremella

作　　者：王昭晶[1] 罗巅辉[1] 曾亚威 WANG Zhao-jing;LUO Dian-hui;ZENG Ya-wei(Department of Bioengineering and Biotechnology,Huaqiao University,Xiamen 361021,China)

机构地区：[1]华侨大学生物工程与技术系

出　　处：《中国药学杂志》2019年第21期1788-1793,共6页Chinese Pharmaceutical Journal

基　　金：国家自然科学基金项目资助(31201314);华侨大学中青年教师科研提升计划资助(ZQN-PY316)

摘　　要：目的对4种银耳属多糖进行分离纯化,并对其理化特性、微观结构及其抗氧化和抗炎症作用进行研究。方法采用热水提取法及DEAE-Sepharose CL-6B和Sephadex G-100柱色谱分离纯化银耳属多糖,利用高效空间排阻色谱法(HPSEC)、气相色谱法(GC)和红外光谱法(FTIR)研究理化特性,透射电子显微镜(TEM)研究微观结构,实时荧光定量PCR研究抗炎症作用。结果分别从金耳、银耳、脑耳和血耳的水提粗提物中分离纯化得到主要多糖TABW-Ⅱ、TFBW-Ⅱ、TEPW-Ⅱ和TSPW-Ⅰ,它们都是均一组分,相对分子质量分别是26×10^3,11×10^3,21×10^3和356×10^3,均主要由葡萄糖和甘露糖组成。4种银耳属多糖均是线状无分支结构,TSPW-Ⅰ形成更加伸展的链缠绕结构。与维生素C相对比,TSPW-Ⅰ显示出更强的羟自由基和超氧自由基清除作用(P<0.05),EC50值分别是0.127和0.088mg·mL-1。TSPW-Ⅰ对脂多糖(LPS)诱导小鼠巨噬细胞RAW264.7产生炎症因子肿瘤坏死因子(TNF)-α、白细胞介素(IL)-6和环氧化酶(COX)-2的抑制作用最强(P<0.05),在150μg·mL-1质量浓度下,抑制率均超过95%。结论血耳主要多糖TSPW-Ⅰ与其他3种银耳属多糖相比拥有不同的糖原组成,相对分子质量大和更加伸展的线状缠绕链结构,同时也表现出最好的抗氧化与抗炎症作用。银耳属多糖都具有抗炎症因子表达的作用,可以作为潜在的抗炎药物开发。OBJECTIVE To isolate four polysaccharides from Tremella and study their physicochemical properties,microstructures,antioxidant activities and anti-inflammation effects.METHODS The polysaccharides were purified using water extraction method,DEAE-Sepharose CL-6B and Sephadex G-100.The physicochemical properties and microstructures were studied using high-performance size-exclusion chromatography(HPSEC),gas chromatography(GC),Fourier transform infrared spectroscopy(FTIR)and transmission electron microscopy(TEM)analysis.Anti-inflammation activities of the polysaccharides were identified using real time quantitative PCR.RESULTS Four kinds of major polysaccharides(TABW-Ⅱ,TFBW-Ⅱ,TEPW-Ⅱand TSPW-Ⅰ)were purified from water-extraction crude polysaccharides from Tremella.TABW-Ⅱ,TFBW-Ⅱ,TEPW-Ⅱand TSPW-Ⅰwere homogeneous with relative molecular mass 26×10^3,11×10^3,21×10^3 and 356×10^3,respectively,and they consisted mainly of glucose and mannose.TEM reveale that their molecular morphologies were linear,and TSPW-Ⅰhad more extended structure with entangled chains.Compared with vitamin C(Vc),TSPW-Ⅰshowed more noticeable scavenging effects of superoxide(EC50=0.127 mg·mL-1)and hydroxyl(EC50=0.088 mg·mL-1)radicals(P<0.05).TSPW-Ⅰalso showed the highest inhibitory effects of TNF-α,IL-6 and COX-2 mRNA expressions in LPS-stimulated RAW 264.7 mouse macrophage cells,and the inhibitory effects were more than 95%at 150μg·mL-1 TSPW-Ⅰ.CONCLUSION The results indicate that TSPW-Ⅰwith entangled and extended liner structures have more obvious antioxidant and anti-inflammation effects than other major polysaccharides from Tremella family.Furthermore,this study indicates the potential utilization of Tremella polysaccharides in anti-inflammatory agents.

关 键 词：银耳属 抗氧化活性 抗炎作用 微观结构 

分 类 号：R965[医药卫生—药理学] Q53[医药卫生—药学]