超薄切片不同制备方法和茶代铀进行心肌淀粉样变性的电子染色  

Electron staining of myocardial amyloidosis by different preparation methods and tea instead of uranium

作　　者：赵然旭[1] 魏英杰[1] 李莉[2] 王清峙[2] 陆洋[2] 徐红宇[2] 有一伟 ZHAO Ranxu;WEI Yingjie;LI Li;WANG Qingzhi;LU Yang;XU Hongyu;YOU Yiwei(Department of Electron Microscopy Platform of National Key Laboratory of Cardiovascular Diseases,Chinese Academy of Medical SciencesPeking Union Medical CollegeNational Center for Cardiovascular Diseases Fuwai Hospital,Beijing 100037,China;Department of Pathology,Chinese Academy of Medical Sciences Peking Union Medical College National Center for Cardiovascular Diseases Fuwai Hospital,Beijing 100037,China)

机构地区：[1]中国医学科学院北京协和医学院国家心血管病中心阜外医院心血管疾病国家重点实验室电镜平台,北京100037 [2]中国医学科学院北京协和医学院国家心血管病中心阜外医院病理科,北京100037

出　　处：《中国医药导报》2019年第28期17-20,F0004,共5页China Medical Herald

基　　金：中国医学科学院医学与健康科技创新工程项目（2016-I2M-1-015）

摘　　要：目的检测乌龙茶提取物是否可作为电子染色剂取代醋酸双氧铀用于心肌淀粉样变染色,并在染色过程当中使用不同的染色方法,改进制备方法,比较其安全性与高效性。方法收集中国医学科学院阜外医院2017年1~12月临床怀疑淀粉样变性的病例共计40例,其中心肌组织经病理学观察和特殊染色呈阳性者10例。利用树脂812常规包埋和改进后方法对心肌组织进行制备,再在复染铅与不复染铅这两种情况下对超薄切片样品进行两种不同染色剂(3%醋酸双氧铀水溶液、0.5%乌龙茶提取物)和两种不同染色方法(滴染法、切片盒染色法)的电子染色,然后在透射电子显微镜下观察比较其不同之处。结果复染铅的情况下,在透射电子显微镜下心肌中淀粉样物质,利用2种电子染色剂和改进后的制备方法进行染色的结果均较理想,切片背景干净,对比度高,所获图像清晰。结论在观察心肌淀粉样变中,乌龙茶提取物配合切片盒染色与改进后的制备方法,可以更加安全、快速地替代醋酸双氧铀进行透射电子显微镜电子染色。Objective To test whether oolong tea extract can be used as an electronic stain instead of uranyl acetate(UA)for myocardial amyloidosis staining.In the process of staining,different staining methods were used and improve the preparation methods and compare their safety and efficiency.Methods A total of 40 cases of clinically suspected amyloidosis were collected from Fuwai Hospital of the Chinese Academy of Medical Sciences from January to December 2017,and 10 cases of cardiac tissue positive after pathological observation and special staining.The myocardial tissues were prepared by conventional encapsulation and improvement of resin 812,and then two kinds of different stains(3% uranyl acetate solution,0.5% oolong tea extract)were applied to the ultrathin section samples under the conditions of counterstaining lead or not counterstaining lead,and two different staining methods(drip method,slice box staining)electronic staining,and then the differences were compared under a transmission electron microscope.Results Under the condition of repeated staining of lead,the results of staining of amyloid substance in myocardium with two kinds of electron staining agents and the improved preparation method were all satisfactory.The background of the slice was clean,the contrast was high and the obtained image was clear.Conclusion In the observation of myocardial amyloidosis,oolong tea extract with section box staining and the improved preparation method can more safely and quickly replace dioxide-uranium acetate for transmission electron microscope electron staining.

关 键 词：乌龙茶提取物 醋酸双氧铀 心肌淀粉样变 透射电子显微镜 

分 类 号：R737.14[医药卫生—肿瘤]