AFP基因多态性与肝细胞癌易感性的相关性研究  

Research on the correlation of AFP gene polymorphism with the susceptibility of hepatocellular carcinoma

作　　者：黄玉亮 吴君荣[1] 赵惠柳[1] 刘志民[1] 梁艺华[1] 黄昭东[1] 朱波[1] HUANG Yuliang;WU Junrong;ZHAO Huiliu;LIU Zhimin;LIANG Yihua;HUANG Zhaodong;ZHU Bo(Department of Clinical Laboratory,the Affiliated Tumor Hospital of Guangxi Medical University,Guangxi Zhuang Autonomous Region,Nanning 530021,China)

机构地区：[1]广西医科大学附属肿瘤医院检验科

出　　处：《中国医药导报》2019年第28期25-29,共5页China Medical Herald

基　　金：国家自然科学基金资助项目（81760530）;广西自然科学基金资助项目（2012GXNSFAA053170、2012GXNS FBA198047）

摘　　要：目的探讨甲胎蛋白(AFP)基因的单核苷酸多态性及血清AFP水平与原发性肝细胞癌(HCC)发病风险的关系。方法纳入2017年7~12月在广西医科大学附属肿瘤医院的206例患者为研究对象,其中HCC患者118例(HCC组),非肿瘤患者88例(对照组)。采用化学发光免疫分析法检测血清AFP水平,应用SNaPshot技术对AFP基因rs4024和rs737241位点多态性进行分型。结果rs7372412位点的基因型和等位基因在HCC组和对照组中的分布频率差异有统计学意义(P<0.05);携带GA或AA基因型的个体患HCC的风险是携带GG基因型的个体的0.543倍(P=0.037,OR=0.543,95%CI:0.306~0.965);携带A等位基因的个体患HCC的风险是携带G等位基因个体的0.573倍(P=0.008,OR=0.573,95%CI:0.379~0.865)。血清AFP水平与年龄、巴塞罗那(BCLC)分期和肝癌病理分级(EDSON)相关(均P<0.05),且年龄越大,血清AFP水平越低(P=0.001,OR=0.172,95%CI:0.059~0.501);BCLC分期越高,血清AFP越高(P=0.000,OR=4.420,95%CI:2.003~9.752);EDSON分级越高,血清AFP越高(P=0.033,OR=2.335,95%CI:1.068~5.102)。结论AFP基因rs737241位点多态性可能与HCC的遗传易感性有关。血清AFP水平可能与年龄和HCC的发展及恶化相关。Objective To investigate the relationship between the single nucleotide polymorphisms of alpha fetal protein(AFP),serum AFP levels and the onset risk of primary hepatocellular carcinoma(HCC).Methods Two hundred and six patients in the Affiliated Tumor Hospital of Guangxi Medical University from July to December 2017 were enrolled,among which,118 cases were HCC patients(HCC group),88 cases were non-tumor patients(control group).The serum AFP levels were detected by chemiluminescence immunoassay,and the polymorphism of rs4024 and rs737241 of AFP gene was analyzed with SNaPshot technique.Results The frequency of genotype and allele at site rs737241 in the HCC group and control group had a significant difference(P<0.05).The risk of HCC in the subjects carrying GA or AA genotype was 0.543 times as big as the individuals carrying GG genotype(P=0.037,OR=0.543,95%CI:0.306-0.965).The onset risk of HCC in individuals carrying A allele was 0.573 times as big as those carrying G allele(P=0.008,OR=0.573,95%CI:0.379-0.865).The serum AFP levels were correlated with age,Barcelona clinic liver cancer(BCLC)stage and Edmondson-Steiner(EDSON)grade(P<0.05),the older the age,the lower the serum AFP levels(P=0.001,OR=0.172,95%CI:0.059-0.501);the higher the BCLC stage,the higher the serum AFP levels(P=0.000,OR=4.420,95%CI:2.003-9.752);the higher the EDSON grade,the higher the serum AFP levels(P=0.033,OR=2.335,95%CI:1.068-5.102).Conclusion The polymorphisms of rs737241 for AFP gene may be correlated with the genetic susceptibility of HCC.The serum AFP level may be correlated with the age,the development and deterioration of HCC.

关 键 词：甲胎蛋白 原发性肝细胞癌 单核苷酸多态性 易感性 

分 类 号：R735.7[医药卫生—肿瘤]