STAT3在猪流行性腹泻病毒感染的作用及机制初步探索  

The preliminary study of the effect and mechanism of STAT3 on PEDV infection

作　　者：杨丽君 张健[1] 许嘉宇 张璐[1] 冯力[1] 陈洪岩[1] 王玉娥[1] YANG Li-jun;ZHANG Jian;XU Jia-yu;ZHANG Lu;FENG Li;CHEN Hong-yan;WANG Yu-e(Division of Laboratory Animal and Comparative Medicine,State Key Laboratory of Veterinary Biotechnology,Harbin Veterinary Research Institute,Chinese Academy of Agriculture Science,Heilongjiang Provincial Key Laboratory of Laboratory Animal and Comparative Medicine,Harbin 150069,China)

机构地区：[1]中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室黑龙江省实验动物与比较医学重点实验室

出　　处：《中国兽医杂志》2019年第7期3-7,共5页Chinese Journal of Veterinary Medicine

基　　金：国家自然科学基金面上项目(31572497)

摘　　要：为探究信号传导及转录激活因子3(Signal transducer and activator of transcription 3,STAT3)在猪流行性腹泻病毒(Porcine epidemic diarrhea virus,PEDV)感染过程中的作用,采用STAT3特异性siRNA处理HEK293和IPEC细胞,作用24 h后,通过荧光定量PCR和Western Blot试验显示,其能够明显降低STAT3的mRNA水平和蛋白水平;在此条件下接种PEDV,通过荧光定量PCR试验,显示当STAT3的mRNA水平降低时能够显著抑制PEDV的RNA水平,Western Blot试验结果也证实抑制STAT3内源性表达能够显著抑制PEDV复制。为进一步研究STAT3的作用机制,采用STAT3特异性siRNA处理这两种细胞,24 h后经过荧光定量PCR检测发现敲低STAT3后,干扰素刺激基因MxA、ISG15以及IFN-β,IFN-λ的mRNA水平均显著升高。本研究对于进一步阐明STAT3参与PEDV感染引发的IFN抗病毒反应提供了试验依据。To investigate the effect of signal transducer and activator of transcription 3(STAT3)on Porcine epidemic diarrhea virus(PEDV)infection,HEK293 and IPEC-J2 cells were transfected with control siRNA or STAT3 specific siRNA for 24 h.By using qRT-PCR and Western Blot,we showed that the expression levels of mRNA and protein of STAT3 were significantly decreased after the treatment.Then the cells were inoculated with PEDV for 24 h and 48 h,respectively and the results showed that knockdown of endogenous STAT3 significantly inhibited PEDV infection.To explore the potential mechanisms,HEK293 and IPEC-J2 cells transfected with control siRNA or STAT3 specific siRNA for 24 h were collected.The results showed that the mRNA levels of MxA,ISG15,IFN-βand IFN-λgenes were significantly increased determined by qRT-PCR.In conclusion,our finding lays a foundation to further clarify the role of STAT3 on PEDV-induced IFN antiviral response.

关 键 词：STAT3 PEDV IFN 

分 类 号：S852.65[农业科学—基础兽医学]