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作 者:李静 王建红 张伊帆 王树伟 LI Jing;WANG Jian-hong;ZHANG Yi-fan(Hebi City Renyuan Blological Technology Development Co.,Ltd.,Hebi,Henan 458030)
机构地区:[1]鹤壁市人元生物技术发展有限公司
出 处:《安徽农业科学》2019年第22期142-145,共4页Journal of Anhui Agricultural Sciences
摘 要:从自然染菌死亡的蚜虫体内分离得到一种杀蚜真菌,经鉴定为寄生曲霉(Aspergillus parasiticus),具有感染流行性,相对湿度75%以上,对蚜虫的侵染率达95%以上,对培养条件进行优化。结果表明,接种量为2.5%(重量比),玉米面和麸皮比例1∶1,水分含量60%,28℃,相对湿度80%,培养时间72 h,产孢量最高达3.0×10^9 CFU/g;该株杀蚜真菌对蚜虫持效期达15 d以上,明显优于10%吡虫啉和40%毒死蜱防治效果。An killing fungus was isolated from aphids that died from natural infection.It was identified as Aspergillus parasitus of aphid fungus,which had infection prevalence.When the relative humidity was above 75%,the infection rate of aphid was above 95%.By optimizing the culture conditions,the test results showed that the inoculation amount was 2.5%(according to the weight ratio of liquid seeds and solid materials);corn flour and bran ratio was 1∶1,moisture 60%,28℃,relative humidity 80%,72 h,the quantity of spore was 3.0×10^9CFU/g;the effort on aphid was more than 15 days,which was obviously better than the control effect of 10%Imidacloprid and 40%Chlorpyrifos.
分 类 号:S433.39[农业科学—农业昆虫与害虫防治]
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