SDF-1联合CGF应用于犬年轻恒牙牙髓样组织再生的研究  

Evaluation of SDF-1 complex with CGF applied to canine dental-pulp-like tissue regeneration

作　　者：乔璐 王慧慧[1] 齐颖 付梦辰 颜燕宏 赵玉梅[1] QIAO Lu;WANG Hui-hui;QI Ying;FU Meng-chen;YAN Yan-hong;ZHAO Yu-mei(Shanghai Engineering Research Center of Tooth Restoration and Regeneration,Department of Pediatric Dentistry,School of Stomatology,Tongji University,Shanghai 200072,China;Jiading District Odontopathy Dispensary,Shanghai 201800,China)

机构地区：[1]上海牙组织修复与再生工程技术中心,同济大学口腔医学院儿童口腔医学教研室,上海200072 [2]上海市嘉定区牙病防治所,上海201800

出　　处：《上海交通大学学报（医学版）》2019年第10期1148-1155,共8页Journal of Shanghai Jiao tong University:Medical Science

基　　金：上海市科学技术委员会科研计划项目（14411963600）~~

摘　　要：目的·探讨以浓缩生长因子(concentrated growth factor,CGF)为支架,联合基质细胞衍生因子1(stromal cell-derived factor-1,SDF-1)应用于年轻恒牙牙髓再生的可行性。方法·体外分离培养犬牙髓细胞(dental pulp cells,DPC),观察SDF-1和CGF对其增殖、分化的影响;建立比格犬年轻恒切牙无髓模型,分为天然牙髓组(A组)、空管组(B组)、填充CGF组(C组)和复合SDF-1/CGF组(D组),10周后行影像学检查、RT-PCR及组织学观察。结果·CGF浸提液(concentrated growth factor extraction,CGFe)促进DPC体外增殖,联合SDF-1可加强其促增殖作用,且可促进细胞成牙、成血管分化;影像学检查示4组根尖均有不同程度生长;RT-PCR示D组新生组织中成牙、成血管相关基因表达均高于C组;组织学观察示C组和D组产生新生牙本质及类牙髓样组织,而B组仅在根尖部产生类牙骨质。结论·CGF可作为年轻恒牙牙髓样组织再生的有效支架,与SDF-1联合应用可促进这一过程。Objective To evaluate the feasibility of immature permanent teeth pulp regeneration with a new method that utilizes the integration of concentrated growth factor(CGF) as a scaffold and stromal cell-derived factor-1(SDF-1). Methods Canine dental pulp cells(DPC) were isolated and cultured in vitro. Then the effects of SDF-1 and CGF were observed on DPC proliferation and differentiation. The pulpless model was established on the beagle’s immature incisors which were divided into four groups: natural pulp(A), empty cannel(B), CGF-filling(C) and SDF-1/CGF-filling(D). After 10 weeks, specimens were checked by imaging examination, RT-PCR and histological observation. Results CGF extraction(CGFe) induced DPC proliferation while the combination of SDF-1 and CGFe enhanced this effect and also facilitated odontogenic and angiogenic differentiation of DPC. According to imaging examination, the apex growth of all four groups was in varying degrees. RT-PCR indicated the expressions of odontogenesis and angiogenesis related genes in group D were higher than those in group C. Besides, neonatal dentin and dental-pulp-like tissue were observed inside the canal of both group C and D, while only cementum-like tissue existed around root apex of group B. Conclusion SDF-1 plays an important role in driving the process of pulp-like-tissue regeneration of immature permanent teeth by using CGF as an effective scaffold.

关 键 词：年轻恒牙 牙髓再生 基质细胞衍生生长因子 浓缩生长因子 

分 类 号：R781.3[医药卫生—口腔医学]