lincRNA-BBOX1-2在胃癌组织中的表达及临床意义  

Expression and clinical significance of long-chain non-coding RNA lincRNA-BBOX1-2 in gastric cancer

作　　者：孙颖[1] 胡梅洁[2] 顾玮[1] 李健[1] 王吉[1] 郑雄[1] SUN Ying;HU Mei-jie;GU Wei;LI Jian;WANG Ji;ZHENG Xiong(Department of Gastroenterology,Luwan Branch,Ruijin Hospital,Shanghai Jiao Tong University School of Medicine,Shanghai 200020,China;Department of Gastroenterology,Ruijin Hospital,Shanghai Jiao Tong University School of Medicine,Shanghai 200025,China)

机构地区：[1]上海交通大学医学院附属瑞金医院卢湾分院消化内科,上海200020 [2]上海交通大学医学院附属瑞金医院消化内科,上海200025

出　　处：《上海交通大学学报（医学版）》2019年第10期1178-1182,共5页Journal of Shanghai Jiao tong University:Medical Science

基　　金：上海市卫生和计划生育委员会科研项目（201540572）~~

摘　　要：目的·研究胃癌组织细胞中长链非编码RNA(long-chain non-coding RNA,lncRNA)lincRNA-BBOX1-2的表达对于胃癌诊断及预后判断的价值。方法·实时定量PCR检测45例胃癌组织及癌旁正常组织中lincRNA-BBOX1-2的表达水平,分析lincRNABBOX1-2的表达水平与胃癌患者临床病理特征及预后的关系。结果·胃癌组织中lincRNA-BBOX1-2的表达水平高于癌旁正常组织(3.291±0.274 vs 1.125±0.075,P=0.000);胃癌组织中lincRNA-BBOX1-2的表达水平与肿瘤有/无淋巴结转移(P=0.005,r=0.172)及TNM分期(P=0.013,r=0.137)呈正相关。受试者工作特征曲线分析结果显示:lincRNA-BBOX1-2在预测胃癌发生时,其曲线下面积(area under the curve,AUC)值为0.916(95%CI 0.859~0.972);lincRNA-BBOX1-2在预测胃癌有/无淋巴结转移时,其AUC值为0.720(95%CI 0.565~0.875)。结论·胃癌组织中lincRNA-BBOX1-2的表达水平上调与胃癌的临床病理特征密切相关。lincRNA-BBOX1-2可能成为一个新的胃癌诊断分子标志物。Objective·To evaluate the expression of long-chain non-coding RNA(lncRNA)lincRNA-BBOX1-2 in gastric cancer tissues and the value of diagnosis and prognosis of gastric cancer.Methods·The expression of lincRNA-BBOX1-2 was detected by real-time quantitative PCR in 45 cases of gastric cancer and adjacent normal tissues.The correlations of lincRNA-BBOX1-2 expression with clinic-pathological features and clinical prognosis were analyzed.Results·The higher expression of lincRNA-BBOX1-2 was observed in the gastric cancer tissues(3.291±0.274 vs 1.125±0.075,P=0.000)as compared with the adjacent normal tissues.Up-regulation of lincRNA-BBOX1-2 was associated with positive lymph node metastasis(P=0.005,r=0.172)and advanced clinical stage(P=0.013,r=0.137).Receiver operating characteristic curve analysis showed that the area under the curve(AUC)value was 0.916(95%CI 0.859–0.972)for lincRNA-BBOX1-2 in predicting the occurrence of gastric cancer,and 0.720(95%CI 0.565–0.875)for lincRNA-BBOX1-2 in predicting the lymph node metastasis.Conclusion·The up-regulation of lincRNA-BBOX1-2 expression in gastric cancer tissues is associated with the clinic-pathological features of gastric cancer.lincRNA-BBOX1-2 may be used as a potential diagnostic biomarker in patients with gastric cancer.

关 键 词：长链非编码RNA lincRNA-BBOX1-2 胃癌 TNM分期 

分 类 号：R735.2[医药卫生—肿瘤]