促红细胞生成素对幼鼠单侧输尿管梗阻解除后肾脏水通道蛋白2-3表达的影响  

Effect of erythropoietin on the expression of aquaporin 2-3 after the release of unilateral ureter obstruction in young rats

作　　者：冯锦锦[1] 闫少华[1] 郭曦 陈燕[1] 文一博[1] 李云龙[1] 何育霖[1] 何翔飞[1] 马源[1] 刘二鹏 宋攀[1] 邢栋 李琦[1] 文建国[1,2] Feng Jinjin;Yan Shaohua;Guo Xi;Chen Yan;Wen Yibo;Li Yunlong;He Yulin;He Xiangfei;Ma Yuan;Liu Erpeng;Song Pan;Xing Dong;Li Qi;Wen Jianguo(Department of Urology&Urodynamic Centre,the First Affiliated Hospital of Zhengzhou University,Zhengzhou 450052,China;Department of Pediatric Urology,the First Affiliated Hospital of Xinxiang Medical University,Weihui 453100,Henan Province,China)

机构地区：[1]郑州大学第一附属医院泌尿外科&小儿尿动力学中心,450052 [2]新乡医学院第一附属医院小儿外科,河南卫辉453100

出　　处：《中华实用儿科临床杂志》2019年第21期1653-1657,共5页Chinese Journal of Applied Clinical Pediatrics

基　　金：国家自然科学基金(81370869)。

摘　　要：目的探讨促红细胞生成素(EPO)对幼鼠单侧输尿管完全梗阻解除(CUUO-R)后肾脏水通道蛋白2-3(AQP2-3)表达的影响。方法选取2~3个月大的SD雄性大鼠24只,随机分为3组:CUUO-R组、CUUO-R+EPO组和假手术组,每组各8只。CUUO-R组和CUUO-R+EPO组行单侧输尿管结扎手术48 h后解除梗阻,CUUO-R+EPO组解除梗阻术后给予EPO腹腔注射(2000 IU/kg),首次于解除梗阻时给药,其余隔天给药1次,共给药4次,CUUO-R组给予同等剂量9 g/L盐水。假手术组仅行输尿管游离,不结扎。解除梗阻术后1周取出肾脏标本,采用免疫组织化学和Western blot的方法检测肾脏中AQP2-3蛋白表达的变化。结果假手术组大鼠尿液渗透压最高,CUUO-R+EPO组次之,CUUO-R组最低(P=0.007)。假手术组的血肌酐和尿素氮分别为(54.820±1.536)μmol/L和(6.929±0.604)mmol/L,CUUO-R+EPO组分别为(57.072±2.286)μmol/L和(1.479±0.043)mmol/L,CUUO-R组分别为(58.001±2.416)μmol/L和(9.025±1.158)mmol/L;组间比较,CUUO-R组与假手术组相比差异有统计学意义(P<0.05),CUUO-R+EPO组与假手术组之间差异无统计学意义(P>0.05)。免疫组织化学结果显示CUUO-R组内髓集合管AQP2及AQP3阳性染色均弱于CUUO-R+EPO组和假手术组,经Western blot检测结果进一步证实,CUUO-R组AQP2和AQP3蛋白的表达量均低于CUUO-R+EPO组和假手术组(AQP2结果分别为0.974±0.109、1.923±0.097和2.002±0.044,F=392.4,P=0.000;AQP3结果分别为0.941±0.048、1.497±0.043和1.863±0.043,F=735.8,P=0.000)。结论EPO对CUUO-R幼鼠AQP2-3蛋白表达和早期肾功能的恢复有益。Objective To investigate the effect of erythropoietin(EPO)on the expression of aquaporin 2-3 after the release of unilateral ureter obstruction in young rats.Methods Twenty-four SD rats were randomly divided into 3 groups(CUUO-R group,CUUO-R+EPO group and sham group,with 8 rats in each group).The CUUO-R model was built through unilateral ureteral ligation,after 48 h the obstruction was released.EPO was given to the CUUO-R+EPO group at the time point of removing obstruction,and then repeated every other day for 1 week,and the same volume of saline was simultaneously given to the CUUO-R rats.The rats in sham group experienced the laparotomy and free dissection of left ureter but not ligation.The kidneys were harvested 7 d after the release of CUUO.The methods of Western blot and immunohistochemistry were used to examine the effects of erythropoietin on the expression of AQP2 and AQP3.Results The osmotic pressure of CUUO-R+EPO group was higher than those of CUUO-R group,but lower than that of sham group(P=0.007).The concentration of creatinine and urea in the CUUO-R group[(58.001±2.416)μmol/L and(9.025±1.158)mmol/L]were higher than those of CUUO-R+EPO group[(57.072±2.286)μmol/L and(1.479±0.043)mmol/L]and sham group[(54.820±1.536)μmol/L and(6.929±0.604)mmol/L].The differences of the concentration of creatinine and urea between CUUO-R group and sham group were statistically significant(P<0.05).There was no significant difference between CUUO-R+EPO group and Sham group(P>0.05).The immunohistochemistry showed that the expressions of AQP2 and AQP3 in co-llecting duct in CUUO-R group were significantly weaker than those of in sham group,and the expression of those in CUUO-R+EPO group were slightly weaker than sham group.These results were further confirmed by Western blot,as the relative quantity of AQP2 and AQP3 were also the lowest in CUUO-R group(AQP2 in 3 groups were 0.974±0.109,1.923±0.097 and 2.002±0.044,F=392.4,P=0.000;AQP3 in 3 groups were 0.941±0.048,1.497±0.043 and 1.863±0.043,F=735.8,P=0.000).Con

关 键 词：促红细胞生成素 输尿管梗阻 水通道蛋白2-3 大鼠 

分 类 号：R73[医药卫生—肿瘤]