miR-224对宫颈癌SiHa和HeLa细胞增殖、凋亡及迁移的影响  

Effects of miR-224 on proliferation,apoptosis and migration of cervical cancer SiHa and HeLa cells

作　　者：张淑萍 杨秀玮[1] Zhang Shuping;Yang Xiuwei(The First People's Hospital of Kashgar,Xinjiang Kashgar 844000,China)

机构地区：[1]喀什地区第一人民医院

出　　处：《现代肿瘤医学》2019年第23期4145-4150,共6页Journal of Modern Oncology

基　　金：新疆维吾尔自治区自然科学基金项目(编号:H201702046)

摘　　要：目的:探讨miR-224对宫颈癌SiHa和HeLa细胞增殖、凋亡及迁移的影响。方法:对宫颈癌细胞株SiHa和HeLa中miR-224进行过表达或沉默,采用qRT-PCR法检测miR-224的转染效果,转染成功后采用CCK-8法、FCM检验、Transwell试验检测各组细胞增殖、凋亡及迁移情况。结果:与正常293T细胞相比,宫颈癌细胞SiHa、HeLa中miR-224表达量均升高,差异具有统计学意义(P<0.01);转染成功后CCK-8法检测SiHa和HeLa细胞增殖情况,与阴性对照组和空白组比较,miR-224 mimic组细胞增殖能力增强,miR-224 inhibitor组细胞增殖能力减弱,差异具有统计学意义(P<0.01);流式细胞仪检测SiHa和HeLa细胞凋亡情况,miR-224 mimic组细胞凋亡百分比明显低于阴性对照组(P<0.01),miR-224 inhibitor组细胞凋亡百分比明显高于阴性对照组(P<0.01);划痕试验检测SiHa和HeLa细胞迁移能力,48 h时miR-224 mimic组细胞愈合速率明显较阴性对照组和空白组快,差异有统计学意义(P<0.01),miR-224 inhibitor组细胞愈合速率明显较阴性对照组慢,差异有统计学意义(P<0.01)。结论:宫颈癌细胞中miR-224过表达可促进细胞增殖和迁移并抑制凋亡,沉默miR-224可抑制细胞增殖和迁移并促进凋亡。Objective:To investigate the effects of miR-224 on proliferation,apoptosis and migration of SiHa and HeLa cells in cervical cancer.Methods:Overexpression or silencing of miR-224 in SiHa and HeLa cervical cancer cell lines was performed,and the transfection effect of miR-224 was detected by qRT-PCR.After successful transfection,CCK-8 method,FCM test and Transwell test were used to detect cell proliferation,apoptosis and migration in each group.Results:Compared with normal 293 T cells,the expressions of miR-224 in cervical cancer cells SiHa and HeLa were both increased,and the difference was statistically significant(P<0.01).After successful transfection,CCK-8 assay was used to detect the proliferation of SiHa and HeLa cells.Compared with the negative control group and the blank group,the proliferation capacity of cells in the miR-224 mimic group was increased,while the proliferation capacity of SiHa and HeLa cells in the miR-224 inhibitor group was decreased,and the difference was statistically significant(P<0.01).Apoptosis of SiHa and HeLa cells was detected by flow cytometry.The percentage of apoptosis in miR-224 mimic group was significantly lower than that in negative control group(P<0.01),and the percentage of apoptosis in SiHa and HeLa cells in miR-224 inhibitor group was significantly higher than that in negative control group(P<0.01).The migration ability of SiHa and HeLa cells was detected by scratch test.At 48 h,the cell healing rate in the miR-224 mimic group was significantly faster than that in the negative control group and the blank group,and the difference was statistically significant(P<0.01).The healing rate of miR-224 inhibitor group was significantly slower than that of the negative control group(P<0.01).Conclusion:Overexpression of miR-224 in cervical cancer cells can promote its proliferation and migration and inhibit apoptosis,while silence of miR-224 can inhibit its proliferation and migration and promote apoptosis.

关 键 词：miR-224 宫颈癌 SIHA细胞 HELA细胞 增殖 凋亡 迁移 

分 类 号：R737.33[医药卫生—肿瘤]