中品661 EMS诱变后代高蛋白的选择效果及新种质创制  

The Selection Effect and Germplasm Innovation for High Protein Content on EMS Mutated Zp661 Progenies

作　　者：魏中艳 李忠峰 刘章雄[1] 邱丽娟[1] WEI Zhong-yan;LI Zhong-feng;LIU Zhang-xiong;QIU Li-juan(Institute of Crop Sciences,Chinese Academy of Agricultural Sciences/The National Key Facility for Crop Gene Resources and Genetic Improvement(NFCRI)/Key Laboratory of Crop Gene Resource and Germplasm Enhancement(MOA),Beijing 100081;College of Agronomy,Henan Agricultural University,Zhengzhou 450046)

机构地区：[1]中国农业科学院作物科学研究所/农业部作物基因资源与遗传改良重大科学工程/农业部作物基因资源与种质创制重点实验室,北京100081 [2]河南农业大学农学院,郑州450046

出　　处：《植物遗传资源学报》2019年第6期1579-1587,共9页Journal of Plant Genetic Resources

基　　金：中国农业科学院科技创新工程项目;大豆种质资源保护与利用(2019NWB036-05);国家农作物资源共享平台(NICGR2019)~~

摘　　要：大豆是重要的植物蛋白来源,高蛋白大豆种质创新对于我国食用大豆育种及优异基因发掘具有重要意义。为获得优异高蛋白大豆资源,本研究利用NIR法测定EMS诱变中品661(Zp661)M2~M7籽粒的蛋白质含量,系统分析连续选择对蛋白变异的影响,旨在创制高蛋白优异种质,为大豆品质改良育种提供优异资源。结果表明,1971个M2单株籽粒蛋白质含量变异幅度为34.96%~50.16%,平均值为42.97%,较野生型(40.88%)提高5.11%,其中17.65%的M2单株籽粒蛋白质含量高于45%。M2高蛋白含量个体经过定向连续选择,整体呈下降趋势;相比之下,M2低蛋白含量个体定向选出的高蛋白含量种质遗传增益效果明显。在M7获得9个高蛋白含量稳定种质(m1~m9),平均蛋白质含量为48.17%,较野生型(41.19%)平均提高16.94%,遗传增益为7.23%。其中m1~m7来自M2高蛋白质含量个体20722(47.21%),而m8~m9来自M2低蛋白质含量个体3442(38.51%)。这些高蛋白纯合种质的农艺性状相关性分析发现,m2、m4和m5的蛋白质含量分别与百粒重和单株粒重呈显著正相关,说明通过选择可以实现蛋白质含量与产量的同时提高,获得高产优质的优异种质。Soybean is an important source of vegetable protein,and innovation of high-protein soybean germplasm becomes of importance in breeding for elite soybean varieties and exploring the underlying mechanism.In order to obtain the high-protein soybean germplasm resources,the protein content of EMS mutagenized″Zp661″M2 population were measured following the NIR(Near-Infrared Reflectance)method,and the subsets of selected offspring lines were further investigated.The results showed that the protein content of 1971 M2 seeds ranged from 34.96% to 50.16% with an average of 42.97%,being 5.11%higher than wild type(40.88%).The protein content in 17.65%of tested M2 samples was higher than 45%.The mutants with high protein content showed a constant reduction in offspring lines in each of generations,whereas an increasing protein content was also observed in offspring lines of M2 mutants with low-protein content.Notably,we identified nine high protein M7 homozygous mutants(m1-m9)with a mean of 48.17%,which were 16.94% higher than wild type(41.19%),with an increased 7.23% genetic gain.The mutants m1 to m7 were derived from sergeants of the M2 high-protein content mutant 20722(47.21%),while m8 and m9 were derived from the M2 low protein content mutant 3442(38.51%).Furthermore,that protein content in m2,m4 and m5 was found to be significantly positively correlated with 100-seed weight and single plant seed yield.Thus,the findings implied no negative correlation between protein content and yield production in some of mutants,and these germplasm resources might become valuable in future breeding for soybean varieties with simultaneous high-yield and high-quality.

关 键 词：大豆 蛋白质含量 甲基磺酸乙酯 遗传变异 

分 类 号：S51[农业科学—作物学]