巨噬细胞的自噬和极化抑制其泡沫化进程  

Autophagy and Polarization of Macrophage Inhibit Foaming Process

作　　者：樊瑛[1] 张秀坤[2] 林朝兰 宫甜甜[3] 王琪[3] 孙瑞珍[3] FAN Ying;ZHANG Xiu-kun;LIN Chao-lan;GONG Tian-tian;WANG Qi;SUN Rui-zhen(Department of Cardiology,First Affiliated Hospital of Harbin Medical University,Harbin,Heilongjiang,150007,China;Hospitals of Heilongjiang Province,Harbin,Heilongjiang,150030,China;Department of Histology and Embryology,Harbin Medical University,Harbin,Heilongjiang,150081,China)

机构地区：[1]哈尔滨医科大学附属第一医院,黑龙江哈尔滨150007 [2]黑龙江省医院,黑龙江哈尔滨150030 [3]哈尔滨医科大学组织胚胎学教研室,黑龙江哈尔滨150081

出　　处：《现代生物医学进展》2019年第19期3621-3625,3669,共6页Progress in Modern Biomedicine

基　　金：黑龙江省教育厅科学技术研究项目(12541450)

摘　　要：目的:巨噬细胞是动脉粥样硬化斑块中最丰富的免疫细胞,巨噬细胞泡沫化加速动脉粥样硬化,本研究探讨巨噬细胞自噬与极化对泡沫化的影响。方法:分离培养小鼠腹腔巨噬细胞,免疫荧光检测巨噬细胞的标记物F4/80。不同浓度雷帕霉素处理巨噬细胞,western blot检测自噬标记物LC3II,经典激活的巨噬细胞(Classically activated macrophages, M1)标记物白细胞介素6(interleukin 6, IL-6)和替代激活的巨噬细胞(Alternatively activated macrophages, M2)标记物转化生长因子β(transform growth factor,TGF-β)的表达。用ox-LDL诱导巨噬细胞泡沫化,油红O染色鉴定泡沫细胞形成及巨噬细胞泡沫化情况。结果:免疫荧光结果显示,小鼠腹腔巨噬细胞F4/80阳性率达87.6%;雷帕霉素处理巨噬细胞24 h,western blot结果显示LC3II表达增加,M1标记物IL-6表达增加,而M2标记物TGF-β表达减少,对其条带进行统计分析结果显示都具有显著性差异(P<0.05);油红O染色结果显示雷帕霉素明显减少巨噬细胞泡沫化形成。结论:雷帕霉素能诱导巨噬细胞自噬,促进其向M1型极化,从而抑制巨噬细胞泡沫化。Objective: Macrophages are the most abundant immune cells in atherosclerotic plaque, and macrophage foaming accelerates atherosclerosis. The study aims to explore the effect of macrophage autophagy and polarization on foaming. Methods: Mouse peritoneal macrophages were isolated and cultured, and the marker F4/80 of macrophages was detected by immunofluorescence.Macrophages were treated with rapamycin at different concentrations. Western blot was used to detect the expression of autophagy markers LC3 II, classically activated macrophages(M1) macrophage markers interleukin 6(IL-6) and alternatively activated macrophages(M2)markers transforming growth factor-β(TGF-β). Macrophages were foamed by ox-LDL, and the foam cells formation and macrophage foaming were identified by oil red O staining. Results: Immunofluorescence assay showed that the positive rate of F4/80 was 87.6%.Western blot showed that increased the expression of LC3 II and IL-6, decreased the expression of TGF-β in rapamycin treatment of macrophages for 24 hours. And, statistical analysis of the bands showed that there were significant differences(P<0.05). Oil red O staining showed that the formation of macrophage foam cells was decreased in rapamycin treatment macrophages. Conclusion: Rapamycin promotes macrophage autophagy and M1 polarization to inhibit foam cell formation.

关 键 词：巨噬细胞 自噬 极化 动脉粥样硬化 

分 类 号：R-33[医药卫生] R543.5