5-氨基酮戊酸声动力疗法诱导人骨肉瘤细胞凋亡的研究  

5-Aminolevulinic Acid Sonodynamic Therapy Induces Apoptosis of Human Osteosarcoma Cells

作　　者：钱贵宾[1] 李卫[2] 姜明久[1] 陈晓颖[3] 王鹏[1] QIAN Gui-bin;LI Wei;JIANG Ming-jiu;CHEN Xiao-ying;WANG Peng(The fourth affiliated hospital ofHarbin Medical University,Harbin,Heilongjiang,150001,China;The first hospital of Harbin City,Harbin,Heilongjiang,150001,China;The first affiliated hospital of Harbin Medical University,Harbin,Heilongjiang,150001,China)

机构地区：[1]哈尔滨医科大学附属第四医院,黑龙江哈尔滨150001 [2]哈尔滨市第一医院,黑龙江哈尔滨150001 [3]哈尔滨医科大学附属第一医院,黑龙江哈尔滨150001

出　　处：《现代生物医学进展》2019年第20期3823-3826,3844,共5页Progress in Modern Biomedicine

基　　金：哈尔滨市科技创新人才专项资金项目(青年后备人才计划类)(2016RAQXJ211);黑龙江省卫生计生委科研基金项目(2016-196;2017-153)

摘　　要：目的:探讨5-氨基酮戊酸声动力疗法(5-ALA-SDT)对人骨肉瘤细胞株U2-OS细胞凋亡的影响。方法:对数生长期的U2-OS细胞随机分为空白对照组(control)、声敏剂组(5-ALA)、超声组(ultrasound),声动力组(5-ALA-SDT)。采用流式细胞术检测细胞凋亡率,荧光探针及共聚焦激光扫描显微镜检测细胞内荧光定位,用DCFH-DA检测活性氧(ROS)的产生情况。结果:与单纯超声组,单纯5-氨基酮戊酸组和对照组相比较,5-氨基酮戊酸介导的声动力组细胞存活率(40.18%±1.35%)均明显下降(P<0.01),细胞内荧光定位表明5-ALA扩散在细胞质和主要累积在U2-OS细胞线粒体内,并且5-氨基酮戊酸介导的声动力组ROS产生的百分率(34.3±2.4)%较单纯超声组、单纯5-氨基酮戊酸组和对照组明显升高,差异有统计学意义(P<0.01)。结论:5-氨基酮戊酸介导的声动力作用可能通过线粒体途径对诱导U2-OS细胞凋亡。Objective: To investigate whether 5-aminoketovaleric acid sonodynamic therapy(5-ALA-SDT)can promote apoptosis of human osteosarcoma cell line u2-os cells and its mechanism. Methods: U2-os cells in logarithmic growth period were randomly divided into control group, acoustic sensitization group(5-ala), ultrasound group(ultrasound), and sonodynamic group(5-ala-sdt).Apoptosis rate was detected by flow cytometry, fluorescence probe and confocal laser scanning microscope were used to detect fluorescence localization in cells, and the production of reactive oxygen species(ROS) was detected by dcfh-da. Results: With simple ultrasound group, pure 5-amino ketones pentanoic acid group compared with control group, 5-amino ketones pentanoic acid mediated the acoustic power group cell survival rate(40.18%+/-1.35%) were significantly decreased(P<0.01), intracellular fluorescence localization showed that 5-ALA diffusion in the cytoplasm and mainly accumulated in U2-OS cell mitochondria, and 5-amino ketones pentanoic acid percentage of ROS mediated the acoustic power group(34.3+2.4) % is relatively simple ultrasound group, 5-pure amino ketones pentanoic acid group and the control group obviously higher. Conclusion: The 5-aminoketovaleric acid mediated sonodynamic effect may induce U2-OS cell apoptosis through the mitochondrial pathway.

关 键 词：声动力疗法 细胞凋亡 骨肉瘤 5-氨基酮戊酸 

分 类 号：R-33[医药卫生] R738