PET-CT示踪加荧光成像监测胰岛及BMSC移植物的分布与定植  

PET-CT tracing and fluorescence imaging to monitor the colonization and distribution of combined transplantation of islets and BMSC

作　　者：魏玲玲 施静 冯天航 赖春友 张恬莹 姚豫桐 邓绍平 黄孝伦 Wei Lingling;Shi Jing;Feng Tianhang;Lai Chunyou;Zhang Tianying;Yao Yutong;Deng Shaoping;Huang Xiaolun(Center for Cell Transplantation,Institute of Organ Transplantation,Sichuan Academy of Medical Sciences&Sichuan Provincial People's Hospital,Chengdu 610072;Molecular Medicine Laboratory,Hospital of Chengdu Office of People's Government of Tibetan Autonomous Region,Chengdu 610041,China)

机构地区：[1]四川省医学科学院四川省人民医院器官移植研究所细胞移植中心,成都610072 [2]西藏自治区人民政府驻成都办事处医院分子医学实验,610041

出　　处：《中华器官移植杂志》2019年第9期527-532,共6页Chinese Journal of Organ Transplantation

基　　金：四川省应用基础研究基金 (2016JY0020);国家重点基础研究发展计划(973计划) (2015CB964703);四川省科技攻关计划 (18ZDYF1208)。

摘　　要：目的利用PET-CT对细胞进行活体示踪,观察移植的胰岛和骨髓间充质干细胞(BMSC)在受鼠体内的分布,间接了解联合移植治疗糖尿病大鼠的疗效。方法链脲佐菌素构建大鼠糖尿病模型,利用胶原酶法分离制备大鼠胰岛,全贴壁法分离培养BMSC,以18F-脱氧葡萄糖(18F-FDG)体外标记。将糖尿病大鼠分组随机法分为四组(每组15只):A为对照组;B为干细胞移植组;C为胰岛移植组;D为联合移植组。均经门静脉移植,PET-CT示踪移植入体内的细胞分布情况。移植后7天,取各组实验鼠肝组织,免疫荧光染色检测移植细胞的定植及分布。应用随机区组方差分析计算标准摄取值(SUV),t检验比较组间差异。结果PET-CT显示,B和D组,BMSC主要均匀分布在右半肝;C和D组,胰岛主要聚集在肝门静脉末端分支;D组,BMSC围绕在胰岛的周围;而对照A组肝脏无明显的显影,PET-CT活体示踪结果与免疫荧光结果一致。结论PET-CT能直观的揭示胰岛与BMSC经门静脉移植后在肝内的分布及相互关系,为临床上胰岛与BMSC联合移植的进一步研究提供了科学依据。Objective To further observe the efficacy of combined transplantation of islet and bone marrow mesenchymal stem cells(BMSC)in diabetic rats,PET-CT was used to trace cells in vivo to determine the homing and distribution of cells in vivo.Methods Streptozotocin(STZ)was used to construct a rat model of diabetes mellitus.BMSC could be isolated and cultured by full adherence method;islets were isolated by collagenase;Islets and BMSC were labeled with 18F-FDG in vitro.Diabetic rats were randomly divided into 4 groups,15 rats in each group:A,Control group;B,Stem cell transplantation group;C,Islet Transplantation group;D,Combined transplantation group,a total of four groups,all transplanted through portal vein,PET-CT tracing the distribution of cells transplanted into the body.7 days after transplantation,the livers of each group were taken,and the homing and distribution of transplanted cells were detected by immunofluorescent staining.The SUV was calculated by the analysis of variance of random block,and the difference between groups was compared by t-test.Results PET-CT results showed that BMSC were mainly distributed uniformly in the right liver,and the islets of the pancreas were mainly clustered in terminal branches of hepatic portal vein,and BMSC were around the islets of pancreas,but there was no obvious development in the liver of the control group.Conclusions PET-CT can directly reveal the distribution of islets and BMSC in liver after transplantation through portal vein.

关 键 词：胰岛移植 骨髓间充质干细胞 正电子发射计算机体层显像仪 

分 类 号：R[医药卫生]