检索规则说明:AND代表“并且”;OR代表“或者”;NOT代表“不包含”;(注意必须大写,运算符两边需空一格)
检 索 范 例 :范例一: (K=图书馆学 OR K=情报学) AND A=范并思 范例二:J=计算机应用与软件 AND (U=C++ OR U=Basic) NOT M=Visual
作 者:张佳丽 杜小弟 吴琼 雷家珩 ZHANG Jia-li;DU Xiao-di;WU Qiong;LEI Jia-heng(School of Chemistry,Chemical Engineering and Life Sciences,Wuhan University of Technology,Wuhan 430070,China)
机构地区:[1]武汉理工大学化学化工与生命科学学院
出 处:《化学试剂》2019年第11期1180-1183,共4页Chemical Reagents
摘 要:优化了甲醇-水流动相HPLC测定银杏萜内酯的方法,建立了线性回归方程,并与药典正丙醇-四氢呋喃-水流动相方法进行比较。在此基础上,用两种方法对银杏叶、银杏外种皮、白果以及银杏叶提取物(EGB)样品中的萜内酯含量进行了对照分析。结果表明,甲醇-水梯度洗脱基线噪音小,柱效高,分离时间短。优化的洗脱条件为:甲醇-水为流动相,0~2 min,0~20%甲醇;2~32 min,20%~60%甲醇,建立的方法在50~2000 mg/L的浓度范围内具有良好的线性关系,检出限为0.05~0.09μg,不同浓度水平的加标回收率为92.4%~101.6%,相对标准偏差(RSD)值为2.7%~5.1%。两种方法测定银杏萜内酯没有明显差别,其中测定银杏叶中白果内酯、银杏内酯C、银杏内酯A、银杏内酯B的相对误差分别为0.86、4.2、1.7、3.9。Optimized HPLC method for determination of terpene lactones in Ginkgo biloba by methanol-water mobile phase.A linear regression equation was established and compared with the pharmacopoeial n-propanol-tetrahydrofuran-water mobile phase method.The content of terpene lactones in Ginkgo leaf,exotestal,seed and extract were compared by these two methods.The results showed the methanol-water gradient elution has lower baseline noise,higher column efficiency and shorter separation time compared with the pharmacopoeia method.The optimized elution conditions:methanol-water as mobile phase,0~2 min,0%~20%methanol;2~32 min,20%~60%methanol.The established method has a good linear relationship in the concentration range of 50~2000 mg/L.The detection limit is 0.05~0.09μg,and the recoveries at different concentration levels is 92.4%~101.6%with RSD 2.7%~5.1%.There was no significant difference in the determination of terpene lactones between these two methods.The relative errors of bibobalide,ginkgolide C,ginkgolide A,and ginkgolide B in Ginkgo leaf were 0.86,4.2,1.7,3.9.
关 键 词:银杏内酯 萜内酯 高效液相色谱-蒸发光散射(HPLC-ELSD) 仪器分析
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在链接到云南高校图书馆文献保障联盟下载...
云南高校图书馆联盟文献共享服务平台 版权所有©
您的IP:216.73.216.222