Lumina B型乳腺癌与肿瘤浸润叉状头/翅膀状螺旋转录因子+调节性T淋巴细胞和CD8+细胞毒性T淋巴细胞预后的关系  

Relationship between prognosis of Lumina B breast cancer and tumor infiltration of forxhead box protein 3+regulatory T lymphocytes and CD8+cytotoxic T lymphocytes

作　　者：彭功玲 王龙强 李强[1] 江学庆[1] Peng Gongling;Wang Longqiang;Li Qiang;Jiang Xueqing(Department of Thyroid and Breast Surgery,the Central Hospital of Wuhan,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430014,China)

机构地区：[1]华中科技大学同济医学院附属武汉中心医院甲状腺乳腺外科,武汉430014

出　　处：《中华实验外科杂志》2019年第11期1975-1977,共3页Chinese Journal of Experimental Surgery

摘　　要：目的检测叉状头/翅膀状螺旋转录因子(FoxP3)和CD8在Lumina B型乳腺癌组织的表达,探讨FoxP3+调节性T淋巴细胞(Treg)和CD8+细胞毒性T淋巴细胞(CTL)与Lumina B型乳腺癌患者预后之间的关系。方法免疫组织化学方法检测乳腺癌组织石蜡切片中FoxP3和CD8在肿瘤间质的表达量;独立样本t检验分析FoxP3和CD8的表达水平与患者年龄、肿瘤大小、淋巴结状态、组织病理分级的关系。Kaplan-Meier Log-rank法分析CD8和FoxP3的表达与乳腺癌患者总体生存率和无病生存率的关系。结果CD8的高表达与肿瘤病灶≤2 cm、淋巴结转移阴性以及较低的病理分级(Ⅰ/Ⅱ级)有关(P<0.05);FoxP3高表达与肿瘤病灶>2 cm、淋巴结转移阳性有关(P<0.01)。另外,FoxP3的高表达明显降低乳腺癌患者的无病生存率(P<0.05),而CD8的高表达提高患者的无病生存率(P<0.01)。结论肿瘤浸润的CD8+CTL与良好预后有关,而肿瘤浸润的FoxP3+Treg与不良预后明显相关。Objective To detect the expression level of forxhead box Protein 3(FoxP3)and CD8 in invasive ductal carcinoma of breast Lumina B,investigate the relevance between FoxP3+regulatory T lymphocyte(Treg)and CD8+cytotoxic T lymphocyte(CTL)as well as prognosis of breast cancer patients.Methods Immunohistochemistry was used to detect the expression level of FoxP3 and CD8 in the paraffin-embedded breast cancer samples.One-sample t test was applied to analyze the relationship among the expression levels of FoxP3 and CD8 of patient’s age,tumor size,nodal status,and histophathologic grade.Kaplan meier Log-rank was used to analyze the correlation between the expression level of CD8 and FoxP3 and the overall survival and disease-free survival of breast cancer patients.Results The expression of CD8 is associated with tumor diameter≤2 cm,lymph node negative metastasis,and with lower histopathologic grade(gradeⅠ/Ⅱ)(P<0.05).The level of FoxP3 is significantly related to tumor diameter>2 cm,lymph node metastasis(P<0.01).High expression level of FoxP3 infiltrating in tumor was could reduce the desease-free survival of breast cancer patients(P<0.05),while low expression of CD8 prolonged desease-free survival(P<0.01).Conclusion Tumor-infiltrating CD8+CTLs are associated with good prognosis,whereas tumor-infiltrating FoxP3+Tregs are associated with adverse outcomes.

关 键 词：乳腺浸润性导管癌 肿瘤浸润淋巴细胞 调节性T淋巴细胞 细胞毒性T淋巴细胞 叉状头/翅膀状螺旋转录因子 CD8 

分 类 号：R[医药卫生]