体外循环对大鼠血管内皮炎症和氧化应激的影响  

Effects of cardiopulmonary bypass on vascular endothelial inflammation and oxidative stress in rats

作　　者：蒋伟[1] 刘建华[1] 石光 梁巧茹[1] Jiang Wei;Liu Jianhua;Shi Guang;Liang Qiaoru(Department of Extracorporeal Circulation of Chest Pain Center,Henan Thoracic Hospital,Henan Zhengzhou,450000)

机构地区：[1]河南省胸科医院胸痛中心体外循环科,郑州450000

出　　处：《中华实验外科杂志》2019年第11期2071-2074,共4页Chinese Journal of Experimental Surgery

基　　金：2018年河南省医学科技攻关计划项目(2018020569)。

摘　　要：目的探讨体外循环(CPB)对大鼠血管内皮炎症和氧化应激的影响及其作用机制。方法64只SD雄性大鼠随机分为假手术组和CPB组,并制备CPB大鼠模型。比较大鼠CPB术后4 h的血管内皮功能;采用酶联免疫吸附试验(ELISA)检测大鼠主动脉血肿瘤坏死因子-α(TNF-α)、白细胞介素(IL)-1β及IL-6含量;采用试剂盒检测大鼠主动脉血丙二醛(MDA)、超氧化物歧化酶(SOD)和谷胱甘肽(GSH)水平;采用蛋白质印迹法(Western blot)检测大鼠主动脉核因子-κB(NF-κB)的蛋白活性。结果与假手术组[(90.17±7.45)%]比较,CPB组大鼠的主动脉血管环舒张度显著下降[(72.57±5.86)%],差异有统计学意义(t=2.869,P<0.05);与假手术组TNF-α、IL-6和IL-1β水平比较[(118.53±9.70)、(87.69±10.46)、(91.88±10.04)ng/L],CPB组大鼠TNF-α、IL-6和IL-1β的血清水平显著上升[(203.55±13.29)、(170.20±11.81)、(176.49±16.11)ng/L],差异有统计学意义(t=3.347、4.253、3.850,P<0.05);与假手术组SOD、MDA和GSH的血清水平比较[(67.38±7.17)U/ml、(21.04±3.7)μmol/L、(20.33±1.79)mg/L],CPB组大鼠SOD和GSH-Px的血清水平显著下降,MDA的血清水平显著增加[(40.38±5.21)U/ml;(34.04±5.0)μmol/L;(13.86±1.51)mg/L],差异有统计学意义(t=2.772、2.397、3.216,P<0.05);Western blot结果显示,与假手术组比较(0.62±0.04),CPB组大鼠主动脉组织中phospho-NF-κB p65的相对表达水平显著增加(1.44±0.12),差异有统计学意义(t=3.750,P<0.05)。结论CPB可通过增强血管内皮炎性反应和氧化应激水平抑制大鼠血管内皮功能。Objective To investigate the effects of cardiopulmonary bypass(CPB)on vascular endothelial inflammation and oxidative stress in rats and its mechanism.Methods 64 SD male rats were randomly divided into sham group and CPB group,and the CPB rat model was prepared.The vascular endothelial function was compared after 4 hours of CPB.tumor necrosis factor-α(TNF-α),interleukin(IL)-1βand IL-6 was detected by enzyme linked immunosorbent assay(ELISA).The serum levels of malondialdehyde(MDA),superoxide dismutase(SOD)and glutathione(GSH)was analyzed using MDA,SOD and GSH test kit.The protein activity of nuclear factor-κB(NF-κB)in aorta was detected by Western blotting.Results Compared with the sham group[(90.17±7.45)%],the diastolicity of aortic vascular in CPB group was significantly decreased[(72.57±5.86)%,t=2.869,P<0.05].Compared with the sham group[(118.53±9.70),(87.69±10.46),(91.88±10.04)ng/L],the serum levels of TNF-α,IL-6 and IL-1βin the CPB group increased significantly[(203.55±13.29),(170.20±11.81),(176.49±16.11)ng/L](t=3.347,4.253,3.850,P<0.05).Compared with the sham group[(67.38±7.17)U/ml;(21.04±3.7)μmol/L;(20.33±1.79)mg/L]the serum levels of SOD and GSH in the CPB group were significantly decreased,while the serum level of MDA was significantly increased[(40.38±5.21)U/ml;(34.04±5.0)μmol/L;(13.86±1.51)mg/L,t=2.772,2.397,3.216,P<0.05].Western blotting showed that compared with the sham group(0.62±0.04),the relative expression of phospho-NF-κB p65 in the aorta tissue of the CPB group was significantly increased(1.44±0.12,t=3.750,P<0.05).Conclusion CPB can inhibit vascular endothelial function in rats by enhancing vascular endothelial inflammation and oxidative stress levels.

关 键 词：体外循环 血管内皮 炎症 氧化应激 核因子-ΚB 

分 类 号：R[医药卫生]