miR-139-5p靶向GPR56抑制骨肉瘤细胞增殖、迁移和侵袭的实验研究  

Experimental study of miR-139-5p inhibiting proliferation,migration and invasion of osteosarcoma cells by targeting GPR56

作　　者：赵玉果[1] 陶海云[2] 叶向阳[1] 王海羽 Zhao Yuguo;Tao Haiyun;Ye Xiangyang;Wang Haiyu(Department of Orthopaedics,Nanyang Central Hospital,Henan Nanyang 473000,China;Department of Oncology,Nanyang Central Hospital,Henan Nanyang 473000,China)

机构地区：[1]南阳市中心医院骨一科,河南南阳473000 [2]南阳市中心医院肿瘤科,河南南阳473000

出　　处：《现代肿瘤医学》2019年第24期4329-4333,共5页Journal of Modern Oncology

基　　金：河南省二〇一八年科技发展计划(编号:182102310466)

摘　　要：目的:研究miR-139-5p对骨肉瘤细胞增殖、迁移和侵袭的影响,并探讨其机制。方法:运用qRT-PCR检测软骨肉瘤细胞中miR-139-5p、GPR56的mRNA表达;Western blot检测细胞中GPR56的蛋白表达;将miR-139-5p组(转染miR-139-5p mimics)、miR-NC组(转染miR-NC)、si-NC组(转染si-NC)、si-GPR56组(转染si-GPR56)、miR-139-5p+pcDNA3.1组(miR-139-5p mimics和pcDNA3.1共转染)、miR-139-5p+pcDNA3.1-GPR56组(miR-139-5p mimics和pcDNA3.1-GPR56共转染),均以脂质体法转染至U-2OS细胞;MTT法检测各组细胞的增殖;Transwell检测各组细胞的迁移、侵袭。结果:与人正常成骨细胞hFOB1.19相比,人骨肉瘤细胞U-2OS中miR-139-5p表达显著降低,GPR56表达显著升高(P<0.05)。过表达miR-139-5p、敲减GPR56均可明显抑制U-2OS细胞增殖、迁移、侵袭;GPR56是miR-139-5p的靶点。过表达GPR56可逆转miR-139-5p对U-2OS细胞增殖、迁移、侵袭的抑制作用。结论:miR-139-5p可抑制骨肉瘤细胞增殖、迁移、侵袭,其机制可能与靶向GPR56有关,将可为骨肉瘤的治疗提供新靶点。Objective:To study the effects of miR-139-5p on proliferation,migration and invasion of osteosarcoma cells and explore its mechanism.Methods:qRT-PCR was used to detect the mRNA expression of miR-139-5p and GPR56 in chondrosarcoma cells.Western blot was used to detect the protein expression of GPR56 in cells.The miR-139-5p group(transfected miR-139-5p mimics),miR-NC group(transfected miR-NC),si-NC group(transfected si-NC),si-GPR56 group(transfected si-GPR56),miR-139-5p+pcDNA3.1 group(co-transfected miR-139-5p mimics and pcDNA3.1),miR-139-5p+pcDNA3.1-GPR56(co-transfected miR-139-5p mimics and pcDNA3.1-GPR56),all transfected to U-2OS cells by liposome method.MTT assay was used to detect the proliferation of each group.Transwell was used to detect the migration and invasion of each group.Results:Compared with human normal osteoblasts hFOB1.19,the expression of miR-139-5p was significantly decreased and the expression of GPR56 was significantly increased in human osteosarcoma cell U-2OS(P<0.05).Overexpression of miR-139-5p and knockdown of GPR56 significantly inhibited proliferation,migration and invasion of U-2OS cells.GPR56 was a target of miR-139-5p.Overexpression of GPR56 reversed the inhibitory effect of miR-139-5p on proliferation,migration and invasion of U-2OS cells.Conclusion:miR-139-5p can inhibit the proliferation,migration and invasion of osteosarcoma cells.The mechanism may be related to targeting GPR56,which will provide a new target for the treatment of osteosarcoma.

关 键 词：miR-139-5p GPR56 骨肉瘤 增殖 迁移 侵袭 

分 类 号：R738.1[医药卫生—肿瘤]