阿糖胞苷对急性白血病细胞中cFLIP表达的影响  

Influence of Ara-C on expression of cFLIP in primary acute leukemic cells

作　　者：赵瑞 尹青松 张文林 刘延方[2] Zhao Rui;Yin Qingsong;Zhang Wenlin;Liu Yanfang(Hematology Department,Zhengzhou University Affiliated Oncology Hospital,Henan Zhengzhou 450008,China;Hematology Department,Zhengzhou University First Affiliated Hospital,Henan Zhengzhou 450008,China)

机构地区：[1]郑州大学附属肿瘤医院血液科,河南郑州450008 [2]郑州大学第一附属医院血液科,河南郑州450008

出　　处：《现代肿瘤医学》2019年第24期4445-4447,共3页Journal of Modern Oncology

基　　金：河南省医学科技攻关计划省部共建项目(编号:201701028)

摘　　要：目的:研究在不同类型初治急性白血病细胞中 cFLIP基因的表达以及阿糖胞苷(Ara-C)对其表达 的影响。方法:选取 36例初治的急性白血病患者骨髓,分离单个核细胞在体外培养,分成两组:未处理组、Ara -C处理组,同时选取 10例非恶性血液病患者的骨髓并分离单个核细胞作为对照组,培养 48小时后,采用逆 转录 PCR(RT-PCR)法检测 cFLIP基因的表达水平。结果:cFLIP在正常人骨髓细胞中不表达或低表达,急 性白血病患者骨髓细胞的 cFLIP表达水平增高(P<0.05);cFLIP在不同类型急性白血病之间的表达无显著 统计学差异(P>0.05);Ara-C可使初治急性白血病患者骨髓细胞的 cFLIP表达水平明显下降(P<0.05)。 结论:cFLIP表达水平增高可能与急性白血病的发生相关;Ara-C治疗白血病的机制之一可能是通过抑制 cFLIP的表达而促进白血病细胞的凋亡。Objective:To investigate the expression of cFLIP in primary acute leukemic cells and the effect of Ara-C on cFLIP expression in acute leukemic cells.Methods:Bone marrow mononuclear cells(BMMNC)from 36 cases of acute leukemia were cultured in vito in 2 groups:Untreated,Ara-C treated.Meanwhile,BMMNC from 10 cases of health adult as control group.After cultured for 48 h,the expression of cFLIP was assessed by RT-PCR.Results:This study found the low expression of cFLIP in the BMMNC of health adult and significantly high expression of cFLIP in the BMMNC of acute leukemia(P<0.05).In vitro culture test,Ara-C down regulated the expression of cFLIP on acute leukemia BMMNC(P<0.05).Conclusion:cFLIP is related to the acute leukemia.The apoptosis is inhibited by cFLIP,which may play an important role in therapy of leukemia by Ara-C.

关 键 词：CFLIP 急性白血病 ARA-C 凋亡 RT-PCR 

分 类 号：R733.71[医药卫生—肿瘤]