OSU-03012对人结肠癌LoVo细胞增殖侵袭及COX-2、VEGF、MMP-2表达的影响  

EFFECTS OF OSU-03012 ON PROLIFERATION AND INVASION OF HUMAN COLON CANCER LOVO CELLS AND THE EXPRESSION OF COX-2, VEGF, MMP-2

作　　者：王虹 WANG Hong(the Seventh People's Hospital of Zhengzhou,Henan Zhengzhou 450000,China)

机构地区：[1]郑州市第七人民医院

出　　处：《承德医学院学报》2019年第6期451-455,共5页Journal of Chengde Medical University

摘　　要：目的:探讨OSU-03012对人结肠癌LoVo细胞增殖、侵袭及环氧合酶-2(COX-2)、血管内皮生长因子(VEGF)、基质金属蛋白酶-2(MMP-2)表达的影响.方法:分别使用0μmol/L、3μmol/L、5μmol/L、10μmol/L的化合物OSU-03012处理人结肠癌LoVo细胞作为研究对象.MTT法检测各组细胞的增殖能力,Transwell法检测各组细胞的侵袭能力,qRT-PCR法和Western Blot法检测各组细胞COX-2、VEGF、MMP-2的表达情况.结果:OSU-03012作用结肠癌LoVo细胞24h、48h和72h,随着药物浓度的增加LoVo细胞的增殖能力逐渐降低,相同时间点不同浓度间两两比较,差异具有统计学意义(P<0.05).不同浓度OSU-03012作用LoVo细胞24h,穿过基底膜的LoVo细胞数量,LoVo细胞COX-2、VEGF、MMP-2 mRNA和蛋白的表达明显降低,不同浓度间两两比较差异有统计学意义(P<0.05).结论:OSU-03012可抑制人结肠癌LoVo细胞的增殖与侵袭,其作用的分子机制可能与下调COX-2、VEGF和MMP-2的表达有关.Objective:To explore the effects of OSU-03012 on proliferation and invasion of human colon cancer LoVo cells and the expression of cyclooxygenase-2(COX-2),vascular endothelial growth factor(VEGF)and matrix metalloproteinase-2(MMP-2).Methods:The human colon cancer LoVo cells were treated with 0μmol/L,3μmol/L,5μmol/L and 10μmol/L of OSU-03012.The proliferation ability of LoVo cells in each group was detected by MTT assay,the invasion ability was detected by Transwell method.The expression of COX-2,VEGF and MMP-2 in LoVo cells were detected by qRT-PCR and Western Blot.Results:The colon cancer LoVo cells were treated with OSU-03012 for 24h,48h and 72h,the proliferation ability of LoVo cells gradually decreased with the increasing of drug concentration;and pairwise comparison of different concentrations at the same time point showed statistically significant differences(P<0.05).After the LoVo cells were treated with OSU-03012 for 24h of different concentrations,the number of LoVo cells passing through the basilar membrane,as well as COX-2,VEGF,MMP-2 mRNA and protein expression decreased significantly;Pairwise comparison of different concentrations showed statistically significant differences(P<0.05).Conclusions:OSU-03012 can inhibit the proliferation and invasion of human colon cancer LoVo cells,and the molecular mechanisms may be related to down-regulation of COX-2,VEGF and MMP-2 expression.

关 键 词：OSU-03012 人结肠癌LoVo细胞 增殖 侵袭 环氧合酶-2(COX-2) 血管内皮生长因子(VEGF) 基质金属蛋白酶-2(MMP-2) 

分 类 号：R735.3[医药卫生—肿瘤]