miR-15a-5p和MDR1在大肠癌组织中的表达及临床意义  

EXPRESSION AND CLINICAL SIGNIFICANCE OF MIR-15A-5P AND MDR1 MRNA IN COLORECTAL CANCER

作　　者：黄艳洁 袁亚男 李璐 许倩[1] 郑纪宁[1] HUANG Yan-jie;YUAN Ya-nan;LI Lu(Chengde Medical College,Hebei Chengde 067000,China)

机构地区：[1]承德医学院

出　　处：《承德医学院学报》2019年第6期455-458,共4页Journal of Chengde Medical University

基　　金：河北省高校省级重点学科建设项目(冀教高(2013)4号病理学与病理生理学)

摘　　要：目的:研究miR-15a-5p和多药耐药基因1(MDR1)mRNA在大肠癌组织中的表达及意义.方法:采用实时荧光定量PCR法检测44例大肠癌组织及对应的癌旁正常肠黏膜组织中miR-15a-5p、MDR1 mRNA的表达情况,分析miR-15a-5p、MDR1 mRNA表达与大肠癌临床病理特征的关系,以及大肠癌组织中miR-15a-5p与MDR1 mRNA表达的相关性.结果:大肠癌组织miR-15a-5p的相对表达量明显低于癌旁正常组织,MDR1 mRNA的相对表达量明显高于癌旁正常组织(P<0.05).大肠癌组织miR-15a-5p、MDR1 mRNA表达均与TNM分期和淋巴结转移有关(P<0.05).Spearman相关分析显示,大肠癌组织miR-15a-5p表达与MDR1 mRNA表达呈负相关关系(r=-0.343,P<0.05).结论:大肠癌组织中miR-15a-5p呈低表达、MDR1呈高表达,二者可能在大肠癌的发生发展过程中发挥重要作用.Objective:To study the expression and clinical significance of miR-15a-5p and multidrug resistance gene 1(MDR1)mRNA in colorectal carcinoma tissues.Methods:RT-qPCR was used to detect the expression of miR-15a-5p and MDR1 mRNA in 44 cases of colorectal carcinoma and adjacent non-tumor tissues.The relationships between miR-15a-5p,MDR1 mRNA and clinical pathological features of colorectal carcinoma,as well as the correlations between miR-15a-5p and MDR1 mRNA in colorectal carcinoma were also analyzed.Results:Compared with the adjacent non-tumor tissues,miR-15a-5p mRNA expression was significantly lower in colorectal carcinoma,but MDR1 mRNA expression was significantly higher(P<0.05).The miR-15a-5p and MDR1 mRNA expression were all concerned with lymph node metastasis and TNM stage of colorectal carcinoma(P<0.05).In colorectal carcinoma,miR-15a-5p mRNA expression was negatively correlated with MDR1 mRNA expression(r=-0.343,P<0.05).Conclusions:The expression of miR-15a-5p was low and MDR1 was high in colorectal cancer tissues.Both of them may play an imoprtant role in development of colorectal carcinoma.

关 键 词：大肠癌 miR-15a-5p 多药耐药基因1(MDR1) 

分 类 号：R735.3[医药卫生—肿瘤]