吲哚-3-甲醇抑制喉癌细胞体外生长的研究  

Indole-3-carbinol inhibits in vitro growth of laryngeal cancer cells

作　　者：毛承刚[1] 周小淳[1] 姜义道[1] 万俐佳[1] 陶泽璋[2] MAO Cheng-gang;ZHOU Xiao-chun;JIANG Yi-dao;WAN Li-jia;TAO Ze-zhang(Department of Otolaryngology,Jingzhou Central Hospital,the Second Clinical Medical College,Yangtze University,Jingzhou 434020,China;Department of Otolaryngology,Renmin Hospital of Wuhan University,Wuhan 430060,China)

机构地区：[1]荆州市中心医院长江大学第二临床医学院耳鼻咽喉科,湖北荆州434020 [2]武汉大学人民医院耳鼻咽喉科,湖北武汉430060

出　　处：《中国耳鼻咽喉颅底外科杂志》2019年第5期504-507,共4页Chinese Journal of Otorhinolaryngology-skull Base Surgery

基　　金：荆州市科技局基金资助项目(2017038;2017044)

摘　　要：目的通过体外实验,研究吲哚-3-甲醇(indole-3-carbinol,I3C)对Hep-2喉癌细胞的抑制增殖和促进凋亡及机制。方法根据I3C浓度分组,用不同浓度的I3C即0、100、200μM和300μM作用于Hep-2喉癌细胞株0、24、48和72 h后,测定Hep-2细胞增殖能力。测定不同浓度I3C处理Hep-2喉癌细胞株48 h后细胞凋亡率。测定不同浓度I3C处理Hep-2喉癌细胞株后PI3K/Akt通路相关蛋白的表达情况。结果当I3C浓度增加时,Hep-2喉癌细胞株的增殖能力明显减弱,凋亡率显著增加,同时监测到PI3K/Akt通路相关蛋白的表达水平降低。结论在体外实验,I3C可以有效地抑制Hep-2喉癌细胞的生长,同时诱导凋亡,抑制PI3K/Akt信号通路可能是其作用机制。Objective To study the inhibition of proliferation and stimulation of apoptosis of Hep-2 laryngeal carcinoma cells by indole-3-carbinol(I3C)via in vitro experiments.Methods Cells were grouped according to the concentration of I3C.The proliferation capacities of Hep-2 cell lines were determined at time-points 0,24,48,and 72h after treatment with I3C at different concentrations of 0,100,200,and 300μM.Their apoptosis rates and expressions of PI3K/Akt pathway related proteins at 48h after treatment with I3C were determined.Results With the increase of I3C concentration,the proliferation abilities of Hep-2 cell lines were significantly decreased with obvious increase of apoptosis rates,and the reduced expression levels of PI3K/Akt pathway related proteins were also observed.Conclusion In vitro,I3C can effectively inhibit the growth of Hep-2 laryngeal cancer cells and induce apoptosis by possible mechanism of inhibiting PI3K/Akt signaling pathway.

关 键 词：喉癌 吲哚-3-甲醇 细胞凋亡 PI3K/AKT通路 

分 类 号：R739.65[医药卫生—肿瘤]