茶油减轻紫外线对小鼠皮肤损伤的作用研究  

The preventive effect of Camellia oil on mouse skin damage induced by UV irradiation

作　　者：张琼[1] 谢璐 陈水亲[2] 宋涛[2] 刘志平[2] 曾招林[3] ZHANG Qiong;XIE Lu;CHEN Shui-qin;SONG Tao;LIU Zhi-ping;ZENG Zhao-lin(Gannan Medical University,Ganzhou,Jiangxi 341000;Gannan Medical University,School of Basic Medicine,Ganzhou,Jiangxi 341000;Gannan Medical University,Department of Dermatology,First Affiliated Hospital,Ganzhou,Jiangxi 341000)

机构地区：[1]赣南医学院,江西赣州341000 [2]赣南医学院基础医学院,江西赣州341000 [3]赣南医学院第一附属医院皮肤科,江西赣州341000

出　　处：《赣南医学院学报》2019年第10期999-1002,共4页JOURNAL OF GANNAN MEDICAL UNIVERSITY

基　　金：国家自然科学基金项目(31560260);江西省教育厅科研项目(GJJ170872,GJJ180802);赣南医学院人才启动基金项目(QD201404);赣南医学院校级油茶专项课题(YC201501);赣南医学院本科生科技创新课题(XS201710);赣南油茶产业开发协同创新中心开放基金项目(YK201603,YK201615)

摘　　要：目的:检测茶油是否能减轻紫外线(UV)照射造成皮肤损伤。方法:以小鼠为研究对象建立UV损伤模型,通过皮肤外用茶油,采用HE和胶原纤维染色的方法确定其是否减轻小鼠皮肤在UV照射后的损伤,同时采用荧光定量PCR方法检测小鼠表皮炎症细胞因子基因的表达。结果:茶油可以减轻小鼠皮肤在UV照射后的损伤,增加胶原纤维的生成,并且可以降低小鼠皮肤促炎性细胞因子IL-6、TNF-α、IL-1β、MCP-1的基因表达水平,差异均有统计学意义(P<0.01)。结论:茶油可以作为防止UV照射的防护用品的候选材料。Objective:To explore if Camellia oil can alleviate the skin damage caused by UV irradiation.Methods:The mouse model of UV injury was established.Camellia oil was applied to the skin,and HE and collagen staining were used to determine whether it could alleviate the damage of the mouse skin after UV irradiation.The effect of Camellia oil on the expression of inflammatory cytokines in skin of mice after UV irradiation was detected using fluorescence quantitative PCR.Results:Camellia oil alleviated the damage of mice skin after UV irradiation,increased the production of collagen fibers,and significantly reduced the gene expression levels of pro-inflammatory cytokines such as IL-6,TNF-α,IL-1β,MCP-1 in mouse skin(P<0.01).Conclusions:Camellia oil can be used as a candidate material to prevent UV irradiation.

关 键 词：茶油 紫外线 皮肤损伤 炎症细胞因子 

分 类 号：R285.5[医药卫生—中药学]